Mark C Fernandez, Glenna J Peterson, Chris Le, Shawn J Skerrett, Thomas R Hawn
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引用次数: 0
摘要
我们研究了 IFNG 是否能在各种条件和方法下限制结核分枝杆菌（Mtb）在人类巨噬细胞中的生长。我们观察到，在四种Mtb菌株的作用下，MCSF分化的单核细胞衍生巨噬细胞（MDMs，4.84 x 105 CFU IFNG刺激与2.48 x 105 CFU未处理相比，P&p;lt;0.001）中的细菌复制会因IFNG而增强。IFNG处理肺泡巨噬细胞不会限制Mtb的复制。与之前的研究一致，IFNG 刺激的小鼠骨髓巨噬细胞有效地限制了 Mtb 的复制。这些数据表明，Mtb在人类巨噬细胞内能抵抗IFNG刺激的免疫，这对不同宿主物种的免疫反应具有影响。
Interferon Gamma Stimulation Fails to Restrict Mycobacterium tuberculosis Growth in Human Monocyte-derived and Alveolar Macrophages
We examined whether IFNG restricts Mycobacterium tuberculosis (Mtb) growth in human macrophages across a range of conditions and methods. We observed an IFNG-dependent enhancement of bacterial replication in MCSF-differentiated monocyte-derived macrophages (MDMs, 4.84 x 105 CFU IFNG stimulated versus 2.48 x 105 CFU untreated, P < 0.001) with four Mtb strains. Mtb replication was not restricted by IFNG treatment of alveolar macrophages. In agreement with previous studies, IFNG-stimulated murine bone marrow derived macrophages effectively restricted Mtb replication. These data suggest that Mtb resists IFNG-stimulated immunity within human macrophages with implications for distinct host species immune responses.
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