母卵抑制成年卵巢转座因子的表达

IF 2.5 3区生物学 Q2 DEVELOPMENTAL BIOLOGY

Developmental biology Pub Date : 2025-04-23 DOI:10.1016/j.ydbio.2025.04.014

Makoto Hayashi , Yuica Koga , Yasuhiro Kozono , Satoru Kobayashi

{"title":"母卵抑制成年卵巢转座因子的表达","authors":"Makoto Hayashi , Yuica Koga , Yasuhiro Kozono , Satoru Kobayashi","doi":"10.1016/j.ydbio.2025.04.014","DOIUrl":null,"url":null,"abstract":"<div><div>In germ cells, repressing transposable elements (TEs) is important to maintain genomic integrity. TE expression and transposition are repressed by PIWI-interacting RNAs (piRNAs). Although many genes for piRNA synthesis have been described, few transcription factors activating their expression have been identified. We previously reported that a transcription factor, maternal Ovo (Ovo-B) protein activates germline-specific gene expression in progenitors of germ cells. In this study, we found that maternal Ovo also activates several genes, including <em>aubergine</em> (<em>aub</em>), for TE silencing. Knocking down maternal Ovo de-repressed TEs in adult ovaries. In addition, embryonic knockdown of <em>aub</em> caused de-repression of TEs in adult <em>Drosophila</em> ovaries. Surprisingly, embryonic knockdown of maternal Ovo affected neither expression of <em>ovo</em> nor its downstream TE-silencing genes in adult ovaries after growth. These results strongly suggest that maternal Ovo is required for TE silencing in ovaries, via transcriptional activation of genes for piRNA synthesis in embryos.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"523 ","pages":"Pages 111-114"},"PeriodicalIF":2.5000,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Maternal ovo represses the expression of transposable elements in adult ovaries\",\"authors\":\"Makoto Hayashi , Yuica Koga , Yasuhiro Kozono , Satoru Kobayashi\",\"doi\":\"10.1016/j.ydbio.2025.04.014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>In germ cells, repressing transposable elements (TEs) is important to maintain genomic integrity. TE expression and transposition are repressed by PIWI-interacting RNAs (piRNAs). Although many genes for piRNA synthesis have been described, few transcription factors activating their expression have been identified. We previously reported that a transcription factor, maternal Ovo (Ovo-B) protein activates germline-specific gene expression in progenitors of germ cells. In this study, we found that maternal Ovo also activates several genes, including <em>aubergine</em> (<em>aub</em>), for TE silencing. Knocking down maternal Ovo de-repressed TEs in adult ovaries. In addition, embryonic knockdown of <em>aub</em> caused de-repression of TEs in adult <em>Drosophila</em> ovaries. Surprisingly, embryonic knockdown of maternal Ovo affected neither expression of <em>ovo</em> nor its downstream TE-silencing genes in adult ovaries after growth. These results strongly suggest that maternal Ovo is required for TE silencing in ovaries, via transcriptional activation of genes for piRNA synthesis in embryos.</div></div>\",\"PeriodicalId\":11070,\"journal\":{\"name\":\"Developmental biology\",\"volume\":\"523 \",\"pages\":\"Pages 111-114\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-04-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Developmental biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0012160625001095\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0012160625001095","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

在生殖细胞中，抑制转座因子（te）对维持基因组完整性至关重要。TE的表达和转位受到piwi相互作用rna （pirna）的抑制。虽然已经描述了许多piRNA合成的基因，但很少有转录因子激活它们的表达。我们之前报道了一种转录因子，母体卵（Ovo- b）蛋白激活生殖细胞祖细胞中生殖系特异性基因的表达。在这项研究中，我们发现母系卵也激活了几个基因，包括茄子（aub），用于TE沉默。在成年卵巢中降低母体卵细胞中去抑制的TEs。此外，胚胎敲低aub可引起成年果蝇卵巢TEs的去抑制。令人惊讶的是，在发育后的成年卵巢中，母体卵细胞的胚胎敲低并不影响卵细胞及其下游te沉默基因的表达。这些结果强烈表明，通过胚胎中piRNA合成基因的转录激活，母体卵是卵巢TE沉默所必需的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Maternal ovo represses the expression of transposable elements in adult ovaries

查看原文本刊更多论文

Maternal ovo represses the expression of transposable elements in adult ovaries

In germ cells, repressing transposable elements (TEs) is important to maintain genomic integrity. TE expression and transposition are repressed by PIWI-interacting RNAs (piRNAs). Although many genes for piRNA synthesis have been described, few transcription factors activating their expression have been identified. We previously reported that a transcription factor, maternal Ovo (Ovo-B) protein activates germline-specific gene expression in progenitors of germ cells. In this study, we found that maternal Ovo also activates several genes, including aubergine (aub), for TE silencing. Knocking down maternal Ovo de-repressed TEs in adult ovaries. In addition, embryonic knockdown of aub caused de-repression of TEs in adult Drosophila ovaries. Surprisingly, embryonic knockdown of maternal Ovo affected neither expression of ovo nor its downstream TE-silencing genes in adult ovaries after growth. These results strongly suggest that maternal Ovo is required for TE silencing in ovaries, via transcriptional activation of genes for piRNA synthesis in embryos.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Developmental biology 生物-发育生物学

CiteScore

5.30

自引率

3.70%

发文量

182

审稿时长

1.5 months

期刊介绍： Developmental Biology (DB) publishes original research on mechanisms of development, differentiation, and growth in animals and plants at the molecular, cellular, genetic and evolutionary levels. Areas of particular emphasis include transcriptional control mechanisms, embryonic patterning, cell-cell interactions, growth factors and signal transduction, and regulatory hierarchies in developing plants and animals.