氯病毒PBCV-1的新结构

IF 2.8 3区医学 Q3 VIROLOGY

Virology Pub Date : 2025-04-22 DOI:10.1016/j.virol.2025.110552

James L. Van Etten , Irina V. Agarkova , David D. Dunigan , Qianqian Shao , Qianglin Fang

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引用次数: 0

摘要

形成大斑块的氯病毒感染真核小球藻样绿藻的分离株。低温电子显微镜（cryo-EM）初步研究表明，PBCV-1为二十面体，多层壳层围绕着一个电子密集的核心，PBCV-1粒子直径约为1900 Å，三角剖分数为169d。然而，正如本综述所述，冷冻电镜技术得到了改进，PBCV-1比最初描述的更复杂。低温电镜（cro - em）图像在8.5 Å下的五倍对称重建显示，该病毒含有一个独特的尖状结构顶点和一个内部单层脂质双层膜。提高到3.5 Å分辨率表明，衣壳包含30个病毒编码的蛋白质，它包含六种不同类型的衣壳体。六种衣壳体中的三种的外表面附着在纤维结构上。

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Emerging structure of chlorovirus PBCV-1

The large plaque-forming chloroviruses infect isolates of eukaryotic chlorella-like green algae. Initial cryo-electron microscopy (cryo-EM) studies revealed that PBCV-1 was icosahedral, with a multilaminate shell surrounding an electron-dense core, and that PBCV-1 particles measured about 1900 Å in diameter with a triangulation number of 169d. However, as described in this review cryo-EM procedures have improved and PBCV-1 is more complex than originally described. A five-fold symmetry reconstruction of cryo-EM images at 8.5 Å revealed that the virus contains a unique vertex with a spike-structure and an internal single lipid bi-layered membrane. Improvement to 3.5 Å resolution revealed that the capsid contains 30 virus-encoded proteins and that it contains six different types of capsomers. The outer surface of three of the six types of capsomers are attached to fiber structures.

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.