鲍曼不动杆菌分离物MAR18-2212产生的K95荚膜多糖含有一种罕见的3-乙酰氨基-3,6-二脱氧-d -葡萄糖（D-Qui3NAc）糖

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2025-04-17 DOI:10.1016/j.carres.2025.109499

Nikolay P. Arbatsky , Alexander S. Shashkov , Nowshin S. Sharar , Fiona J. Baird , Mikhail M. Shneider , Anna M. Shpirt , Andrei V. Perepelov , Andrei S. Dmitrenok , Yulia V. Mikhailova , Andrey A. Shelenkov , Anastasiya V. Popova , Johanna J. Kenyon , Yuriy A. Knirel

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引用次数: 0

摘要

对2018年从俄罗斯人呼吸道标本中分离得到的鲍曼不动杆菌MAR18-2212的全基因组序列进行了分析，发现该菌株在荚膜多糖（CPS）生物合成K位点携带KL95。KL95包括一个由四个基因组成的模块（rmlB-rmlA-qdtE-qdtB），预测3-乙酰氨基-3,6-二脱氧-d-葡萄糖（d-Quip3NAc）的合成。通过二维1Н、1Н COSY、1Н、1Н TOCSY、1Н、1Н ROESY、1Н、13C HSQC和1Н、13C HMBC实验建立了从MAR18-2212中分离得到的K95 CPS的结构，并通过Smith降解得到了证实。K95由支链六糖k单元组成，含有3个d-GalpNAc残基、2个d-Galp残基和1个d-Quip3NAc残基。KL95编码的酶的定位确定了rmlB-rmlA-qdtE-qdtB在d-Quip3NAc合成中的作用。5种编码的糖基转移酶和Wzy聚合酶也被分配到K95的糖苷键上。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The K95 capsular polysaccharide produced by Acinetobacter baumannii isolate MAR18-2212 includes a rarely encountered 3-acetamido-3,6-dideoxy-D-glucose (D-Qui3NAc) sugar

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The K95 capsular polysaccharide produced by Acinetobacter baumannii isolate MAR18-2212 includes a rarely encountered 3-acetamido-3,6-dideoxy-D-glucose (D-Qui3NAc) sugar

The whole genome sequence from Acinetobacter baumannii MAR18-2212, an isolate recovered in 2018 from a human respiratory tract specimen in Russia, was obtained and found to carry KL95 at the capsular polysaccharide (CPS) biosynthesis K locus. KL95 includes a module of four genes (rmlB-rmlA-qdtE-qdtB) predicting synthesis of 3-acetamido-3,6-dideoxy-d-glucose (d-Quip3NAc). The structure of the K95 CPS isolated from MAR18-2212 was established using ¹H and ¹³C NMR spectroscopy, including two-dimensional ¹Н,¹Н COSY, ¹Н,¹Н TOCSY, ¹Н,¹Н ROESY, ¹Н,¹³C HSQC, and ¹Н,¹³C HMBC experiments, and was confirmed by Smith degradation. K95 is composed of branched hexasaccharide K-units containing three d-GalpNAc residues, two d-Galp residues and one residue of d-Quip3NAc. Assignment of the enzymes encoded by KL95 established the role of rmlB-rmlA-qdtE-qdtB in the synthesis of d-Quip3NAc. Five encoded glycosyltransferases and the Wzy polymerase were also assigned to the glycosidic linkages in K95.

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".