跨越发育时间点的人类神经干细胞成熟神经元的转录组学特征

IF 2.9 Q3 NEUROSCIENCES
Kimia Hosseini , Gaëtan Philippot , Sara B. Salomonsson , Andrea Cediel-Ulloa , Elnaz Gholizadeh , Robert Fredriksson
{"title":"跨越发育时间点的人类神经干细胞成熟神经元的转录组学特征","authors":"Kimia Hosseini ,&nbsp;Gaëtan Philippot ,&nbsp;Sara B. Salomonsson ,&nbsp;Andrea Cediel-Ulloa ,&nbsp;Elnaz Gholizadeh ,&nbsp;Robert Fredriksson","doi":"10.1016/j.ibneur.2025.04.013","DOIUrl":null,"url":null,"abstract":"<div><div>Neurodevelopmental studies employing animal models encounter challenges due to interspecies differences and ethical concerns. Maturing neurons of human origin, undergoing several developmental stages, present a powerful alternative. In this study, human embryonic stem cell (H9 cell line) was differentiated into neural stem cells and subsequently matured into neurons over 30 days. Ion AmpliSeq™ was used for transcriptomic characterization of human stem cell-derived neurons at multiple time points. Data analysis revealed a progressive increase of markers associated with neuronal development and astrocyte markers, indicating the establishment of a co-culture accommodating both glial and neurons. Transcriptomic and pathway enrichment analysis also revealed a more pronounced GABAergic phenotype in the neurons, signifying their specialization toward this cell type. The findings confirm the robustness of these cells across different passages and demonstrate detailed progression through stages of development. The model is intended for neurodevelopmental applications and can be adapted to investigate how genetic modifications or exposure to chemicals, pharmaceuticals, and other environmental factors influence neurons and glial maturation.</div></div>","PeriodicalId":13195,"journal":{"name":"IBRO Neuroscience Reports","volume":"18 ","pages":"Pages 679-689"},"PeriodicalIF":2.9000,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transcriptomic characterization of maturing neurons from human neural stem cells across developmental time points\",\"authors\":\"Kimia Hosseini ,&nbsp;Gaëtan Philippot ,&nbsp;Sara B. Salomonsson ,&nbsp;Andrea Cediel-Ulloa ,&nbsp;Elnaz Gholizadeh ,&nbsp;Robert Fredriksson\",\"doi\":\"10.1016/j.ibneur.2025.04.013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Neurodevelopmental studies employing animal models encounter challenges due to interspecies differences and ethical concerns. Maturing neurons of human origin, undergoing several developmental stages, present a powerful alternative. In this study, human embryonic stem cell (H9 cell line) was differentiated into neural stem cells and subsequently matured into neurons over 30 days. Ion AmpliSeq™ was used for transcriptomic characterization of human stem cell-derived neurons at multiple time points. Data analysis revealed a progressive increase of markers associated with neuronal development and astrocyte markers, indicating the establishment of a co-culture accommodating both glial and neurons. Transcriptomic and pathway enrichment analysis also revealed a more pronounced GABAergic phenotype in the neurons, signifying their specialization toward this cell type. The findings confirm the robustness of these cells across different passages and demonstrate detailed progression through stages of development. The model is intended for neurodevelopmental applications and can be adapted to investigate how genetic modifications or exposure to chemicals, pharmaceuticals, and other environmental factors influence neurons and glial maturation.</div></div>\",\"PeriodicalId\":13195,\"journal\":{\"name\":\"IBRO Neuroscience Reports\",\"volume\":\"18 \",\"pages\":\"Pages 679-689\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-04-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IBRO Neuroscience Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667242125000612\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IBRO Neuroscience Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667242125000612","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

由于物种间差异和伦理问题,采用动物模型的神经发育研究遇到了挑战。人类起源的成熟神经元,经历了几个发育阶段,提供了一个强大的选择。在本研究中,人类胚胎干细胞(H9细胞系)分化为神经干细胞,随后在30天内成熟为神经元。使用Ion AmpliSeq™在多个时间点对人类干细胞来源的神经元进行转录组学表征。数据分析显示,与神经元发育相关的标志物和星形胶质细胞标志物逐渐增加,表明胶质细胞和神经元的共培养已经建立。转录组学和途径富集分析也揭示了神经元中更明显的gaba能表型,表明它们向这种细胞类型特化。研究结果证实了这些细胞在不同传代中的稳健性,并展示了发育阶段的详细进展。该模型旨在用于神经发育应用,并可用于研究遗传修饰或暴露于化学品,药物和其他环境因素如何影响神经元和神经胶质成熟。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptomic characterization of maturing neurons from human neural stem cells across developmental time points
Neurodevelopmental studies employing animal models encounter challenges due to interspecies differences and ethical concerns. Maturing neurons of human origin, undergoing several developmental stages, present a powerful alternative. In this study, human embryonic stem cell (H9 cell line) was differentiated into neural stem cells and subsequently matured into neurons over 30 days. Ion AmpliSeq™ was used for transcriptomic characterization of human stem cell-derived neurons at multiple time points. Data analysis revealed a progressive increase of markers associated with neuronal development and astrocyte markers, indicating the establishment of a co-culture accommodating both glial and neurons. Transcriptomic and pathway enrichment analysis also revealed a more pronounced GABAergic phenotype in the neurons, signifying their specialization toward this cell type. The findings confirm the robustness of these cells across different passages and demonstrate detailed progression through stages of development. The model is intended for neurodevelopmental applications and can be adapted to investigate how genetic modifications or exposure to chemicals, pharmaceuticals, and other environmental factors influence neurons and glial maturation.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
IBRO Neuroscience Reports
IBRO Neuroscience Reports Neuroscience-Neuroscience (all)
CiteScore
2.80
自引率
0.00%
发文量
99
审稿时长
14 weeks
期刊介绍:
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信