猴头菌提取物通过激活Nrf2/HO-1通路和调节肠道菌群减轻大鼠胃黏膜损伤

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-04-25 DOI:10.1016/j.jff.2025.106842

Feng Zhu , Hongye Pan , Bin Li , Xiaoping Mao , Zhilin Chen , Guanzeng Ye , Jiawen Gao , Yujia Shen , Jiangning Hu , Zhaohuan Lou

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引用次数: 0

摘要

猴头菌（Hericium erinaceus）是一种著名的药食用菌，广泛用于治疗胃肠道疾病。本研究旨在探讨猴头菌提取物（heicium erinaceus extract， HEE）对胃粘膜病变的防治作用，并探讨其作用机制。在无水乙醇处理大鼠之前，通过灌胃给予HEE。组织学检查和抗氧化能力评估表明，HEE明显减轻了胃粘膜损伤的严重程度，增强了抗氧化酶的活性。定量PCR （qPCR）分析显示，HEE调节了Nrf2信号通路。HEE处理乙醇损伤的GES-1细胞表明，HEE降低丙二醛（MDA）水平，同时增加超氧化物歧化酶（SOD）和谷胱甘肽（GSH）活性。免疫印迹结果显示，HEE能激活Nrf2，提高HO-1蛋白在体外的表达。建立醋酸诱导大鼠模型，观察HEE对胃溃疡的治疗作用。HEE有效减少胃溃疡面积，增强肠道微生物群多样性。上述结果表明，猴头菌提取物是一种潜在的治疗药物，其预防和治疗胃粘膜损伤的机制可能涉及增强抗氧化能力、调节Nrf2通路和调节肠道菌群。

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Hericium erinaceus extract attenuates gastric mucosal injury via Nrf2/HO-1 pathway activation and gut microbiota modulation in rats

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Hericium erinaceus extract attenuates gastric mucosal injury via Nrf2/HO-1 pathway activation and gut microbiota modulation in rats

Hericium erinaceus is a well-known medicinal-edible fungus widely utilized in the treatment of gastrointestinal disorders. This study aims to investigate the preventive and therapeutic effects of Hericium erinaceus extract (HEE) on gastric mucosal lesions and to explore the mechanisms underlying its action. HEE was administered by gavage to rats before they were treated with absolute ethanol. Histological examination and assessment of antioxidant capacity demonstrated that HEE significantly alleviated the severity of gastric mucosal injury and enhanced the activity of antioxidant enzymes. Quantitative PCR (qPCR) analysis revealed that HEE modulated the Nrf2 signaling pathway. HEE treatment of ethanol injured GES-1 cells showed that HEE reduces malondialdehyde (MDA) levels while simultaneously increasing superoxide dismutase (SOD) and glutathione (GSH) activities. Immunoblot results indicated that HEE could activate Nrf2 and increase HO-1 protein expression in vitro. A rat model induced by acetic acid was established to investigate the ability of HEE to treat gastric ulcers. HEE effectively reduced the gastric ulcer area and enhanced gut microbiome diversity. These findings show that Hericium erinaceus extract is a potential therapeutic agent, and its mechanisms for preventing and treating gastric mucosal injury may involve enhancing antioxidant capacity, regulating the Nrf2 pathway and modulating gut microbiota.

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.