抗氧化菌株planplantibacillus plantarum BX62通过调节NLRP3/Caspase-1/IL-1β和AKT/mTOR通路，平衡免疫稳态，缓解类风湿关节炎

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-04-24 DOI:10.1016/j.jff.2025.106832

Qiang Li , Zitong Ding , Hu Chen , Mengya Jia , Samaila Usman , Qi Liu , Mengyan Chen , Tingtao Chen , Azmat Ullah Jan , Qi Qin , Xusheng Guo

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引用次数: 0

摘要

本研究通过胶原诱导的关节炎大鼠模型，探讨抗氧化剂植物乳杆菌BX62 （2×109CFU/ml）对RA的影响，并阐明其作用机制。结果显示，植物乳杆菌BX62可降低氧化应激，降低血清炎症因子水平，提高抗炎白细胞介素-10水平。通过组织学和显微计算机断层扫描分析证实骨损伤减轻。此外，植物乳杆菌BX62还能恢复免疫平衡和脾脏CD4+/CD8+ T细胞比例。L. plantarum BX62组NLRP3、Cleaved caspase -1、Cleaved IL-1β、RANKL、p-AKT、p-mTOR和p62蛋白下调，OPG和Beclin-1蛋白上调。同时，植物乳杆菌BX62稳定的器官系数表明其口服安全。总体而言，L. plantarum BX62通过降低氧化应激、调节NLRP3/Caspase-1/IL-1β、AKT/mTOR通路、平衡自噬和增强骨保护，显示出预防RA进展的作用；因此，使其成为治疗类风湿性关节炎的强大益生菌菌株。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The antioxidant strain Lactiplantibacillus plantarum BX62 alleviates rheumatoid arthritis via balancing immune homeostasis and modulating NLRP3/Caspase-1/IL-1β and AKT/mTOR pathways

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The antioxidant strain Lactiplantibacillus plantarum BX62 alleviates rheumatoid arthritis via balancing immune homeostasis and modulating NLRP3/Caspase-1/IL-1β and AKT/mTOR pathways

This study explored the effect of antioxidant Lactiplantibacillus plantarum BX62 (2×10⁹CFU/ml) on RA and elucidated its underlying mechanisms using a collagen induced arthritis rat model. Results showed L. plantarum BX62 reduced oxidative stress, decreased the levels of inflammatory cytokines in the serum, and increased anti-inflammatory interleukin-10 levels. Bone damage mitigation was confirmed via histology and micro-computed tomography analyses. Furthermore, L. plantarum BX62 restored immune balance and CD4+/CD8+ T cell ratio in the spleen. Downregulations of NLRP3, Cleaved Caspased-1, Cleaved IL-1β, RANKL, p-AKT, p-mTOR, and p62, and upregulations of OPG and Beclin-1 proteins were also observed in L. plantarum BX62 group. Meanwhile, The stable organ coefficients of L. plantarum BX62 demonstrated its safety for oral administration. Overall, L. plantarum BX62 demonstrated preventive effects against RA progression by reducing oxidative stress, modulating NLRP3/Caspase-1/IL-1β, AKT/mTOR pathways, balancing autophagy, and enhancing bone protection; thus, making it a formidable probiotic strain for curing RA.

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.