布鲁氏菌灭活疫苗可引起小鼠和豚鼠对梅利氏杆菌感染的免疫

IF 6.9 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Biomedicine & Pharmacotherapy Pub Date : 2025-04-24 DOI:10.1016/j.biopha.2025.118077

Ruirui Hu , Qianyi Zhang , Wenjia Wang , Wenhao Ren , Mengxin Yao , Yimei Xu , Hui Zhang , Jinliang Sheng , Yong Wang , Chuangfu Chen , Zhongchen Ma

{"title":"布鲁氏菌灭活疫苗可引起小鼠和豚鼠对梅利氏杆菌感染的免疫","authors":"Ruirui Hu , Qianyi Zhang , Wenjia Wang , Wenhao Ren , Mengxin Yao , Yimei Xu , Hui Zhang , Jinliang Sheng , Yong Wang , Chuangfu Chen , Zhongchen Ma","doi":"10.1016/j.biopha.2025.118077","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Brucellosis is a zoonotic disease that poses a significant threat to both animal husbandry and public health. Currently available vaccines for brucellosis are all live attenuated forms, which carry the risk of potential infectivity and provide a relatively limited range of protection. In contrast, inactivated vaccines are perceived to exhibit poor protective efficacy and fail to elicit effective cellular immunity. This study aimed to comprehensively evaluate the efficacy of the <em>Brucella</em> inactivated vaccine (CF) with the objective of developing a safer and more effective candidate for brucellosis vaccination.</div></div><div><h3>Methods</h3><div>Firstly, we evaluated the safety of CF in mice. Subsequently, we immunized mice with CF using various doses and methods, determining the optimal immunization dose and method through challenge testing. We evaluated the vaccine’s immunogenicity by detecting the cellular and humoral immune levels induced by CF in mice, and assessed the vaccine’s protective effect based on the post challenge organ bacterial load. Additionally, we evaluated the protective effects of dual doses and secondary immunization in guinea pigs.</div></div><div><h3>Results</h3><div>The results indicate that CF is safe and non-toxic; it induced significant increases in specific IgG antibody levels against <em>Brucella</em> during the early stages and markedly enhanced the T cell immune response, thereby promoting a Th1-biased immune response in mice. Following the challenge, CF demonstrated protective efficacy comparable to that of the S2 vaccine against <em>B. melitensis</em> biovar 3 infection in mice. CF-immunized guinea pigs were able to resist infection by <em>B. melitensis</em> M28 and <em>B. melitensis</em> biovar 3.</div></div><div><h3>Conclusions</h3><div>In summary, CF significantly induces both humoral and cellular immunity in mice. This study reports for the first time that a safe and effective inactivated <em>Brucella</em> vaccine (CF) can induce cellular immune responses and effectively prevent animal brucellosis.</div></div>","PeriodicalId":8966,"journal":{"name":"Biomedicine & Pharmacotherapy","volume":"187 ","pages":"Article 118077"},"PeriodicalIF":6.9000,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Brucella inactivated vaccine elicits immunity against B. melitensis infection in mice and guinea pigs\",\"authors\":\"Ruirui Hu , Qianyi Zhang , Wenjia Wang , Wenhao Ren , Mengxin Yao , Yimei Xu , Hui Zhang , Jinliang Sheng , Yong Wang , Chuangfu Chen , Zhongchen Ma\",\"doi\":\"10.1016/j.biopha.2025.118077\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Brucellosis is a zoonotic disease that poses a significant threat to both animal husbandry and public health. Currently available vaccines for brucellosis are all live attenuated forms, which carry the risk of potential infectivity and provide a relatively limited range of protection. In contrast, inactivated vaccines are perceived to exhibit poor protective efficacy and fail to elicit effective cellular immunity. This study aimed to comprehensively evaluate the efficacy of the <em>Brucella</em> inactivated vaccine (CF) with the objective of developing a safer and more effective candidate for brucellosis vaccination.</div></div><div><h3>Methods</h3><div>Firstly, we evaluated the safety of CF in mice. Subsequently, we immunized mice with CF using various doses and methods, determining the optimal immunization dose and method through challenge testing. We evaluated the vaccine’s immunogenicity by detecting the cellular and humoral immune levels induced by CF in mice, and assessed the vaccine’s protective effect based on the post challenge organ bacterial load. Additionally, we evaluated the protective effects of dual doses and secondary immunization in guinea pigs.</div></div><div><h3>Results</h3><div>The results indicate that CF is safe and non-toxic; it induced significant increases in specific IgG antibody levels against <em>Brucella</em> during the early stages and markedly enhanced the T cell immune response, thereby promoting a Th1-biased immune response in mice. Following the challenge, CF demonstrated protective efficacy comparable to that of the S2 vaccine against <em>B. melitensis</em> biovar 3 infection in mice. CF-immunized guinea pigs were able to resist infection by <em>B. melitensis</em> M28 and <em>B. melitensis</em> biovar 3.</div></div><div><h3>Conclusions</h3><div>In summary, CF significantly induces both humoral and cellular immunity in mice. This study reports for the first time that a safe and effective inactivated <em>Brucella</em> vaccine (CF) can induce cellular immune responses and effectively prevent animal brucellosis.</div></div>\",\"PeriodicalId\":8966,\"journal\":{\"name\":\"Biomedicine & Pharmacotherapy\",\"volume\":\"187 \",\"pages\":\"Article 118077\"},\"PeriodicalIF\":6.9000,\"publicationDate\":\"2025-04-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedicine & Pharmacotherapy\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0753332225002719\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedicine & Pharmacotherapy","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0753332225002719","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

摘要

布鲁氏菌病是一种人畜共患疾病，对畜牧业和公共卫生构成重大威胁。目前可用的布鲁氏菌病疫苗均为减毒活疫苗，具有潜在传染性风险，提供的保护范围相对有限。相反，灭活疫苗被认为表现出较差的保护功效，不能引起有效的细胞免疫。本研究旨在综合评价布氏菌灭活疫苗（CF）的有效性，以开发一种更安全、更有效的候选布氏菌病疫苗。方法首先对CF在小鼠体内的安全性进行评价。随后，我们采用不同剂量和方法对小鼠进行CF免疫，通过攻毒试验确定最佳免疫剂量和方法。我们通过检测CF诱导小鼠的细胞和体液免疫水平来评估疫苗的免疫原性，并根据攻击后器官细菌负荷来评估疫苗的保护作用。此外，我们评估了双剂量和二次免疫对豚鼠的保护作用。结果CF安全无毒；在早期阶段诱导针对布鲁氏菌的特异性IgG抗体水平显著增加，并显著增强T细胞免疫反应，从而促进小鼠的th1偏倚免疫反应。在攻毒后，CF显示出与S2疫苗对小鼠melitensis生物品种3感染相当的保护功效。猪瘟免疫豚鼠能够抵抗猪白僵菌M28和猪白僵菌生物变种3的感染。综上所述，CF可显著诱导小鼠体液免疫和细胞免疫。本研究首次报道了一种安全有效的布鲁氏菌灭活疫苗（CF）可诱导细胞免疫应答，有效预防动物布鲁氏菌病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Brucella inactivated vaccine elicits immunity against B. melitensis infection in mice and guinea pigs

Background

Brucellosis is a zoonotic disease that poses a significant threat to both animal husbandry and public health. Currently available vaccines for brucellosis are all live attenuated forms, which carry the risk of potential infectivity and provide a relatively limited range of protection. In contrast, inactivated vaccines are perceived to exhibit poor protective efficacy and fail to elicit effective cellular immunity. This study aimed to comprehensively evaluate the efficacy of the Brucella inactivated vaccine (CF) with the objective of developing a safer and more effective candidate for brucellosis vaccination.

Methods

Firstly, we evaluated the safety of CF in mice. Subsequently, we immunized mice with CF using various doses and methods, determining the optimal immunization dose and method through challenge testing. We evaluated the vaccine’s immunogenicity by detecting the cellular and humoral immune levels induced by CF in mice, and assessed the vaccine’s protective effect based on the post challenge organ bacterial load. Additionally, we evaluated the protective effects of dual doses and secondary immunization in guinea pigs.

Results

The results indicate that CF is safe and non-toxic; it induced significant increases in specific IgG antibody levels against Brucella during the early stages and markedly enhanced the T cell immune response, thereby promoting a Th1-biased immune response in mice. Following the challenge, CF demonstrated protective efficacy comparable to that of the S2 vaccine against B. melitensis biovar 3 infection in mice. CF-immunized guinea pigs were able to resist infection by B. melitensis M28 and B. melitensis biovar 3.

Conclusions

In summary, CF significantly induces both humoral and cellular immunity in mice. This study reports for the first time that a safe and effective inactivated Brucella vaccine (CF) can induce cellular immune responses and effectively prevent animal brucellosis.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biomedicine & Pharmacotherapy 医学-药学

CiteScore

11.90

自引率

2.70%

发文量

1621

审稿时长

48 days

期刊介绍： Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.