生成融合标记甲状腺激素受体α亚型的hiPSC细胞系，研究TRα1和TRα2的特异性作用

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-04-17 DOI:10.1016/j.scr.2025.103720

Nina Härting , Katarzyna A. Ludwik , Regina Jahn , Frank J. Kaiser , Harald Stachelscheid

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引用次数: 0

摘要

核甲状腺激素受体TRα1作为甲状腺激素作用靶基因表达的中介的作用已被充分了解。然而，不结合甲状腺激素的TRα2剪接异构体的功能仍未被发现。由于没有可靠的抗体专门研究TRα1和TRα2，我们通过CRISPR/ cas9介导的基因组编辑将小融合标签引入健康男性供体iPSC系BIHi001-B和BIHi005-A的THRA位点。因此，修饰的细胞系表达c端标记的TRα1-2xHA或TRα2-3xFLAG。这些基因组编辑的细胞系有助于研究TRα在不同细胞类型中的异构体特异性作用。

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Generation of hiPSC lines with fusion tagged thyroid hormone receptor α isoforms to study isoform-specific actions of TRα1 and TRα2

The role of the nuclear thyroid hormone receptor TRα1 as a mediator of thyroid hormone action on target gene expression is well understood. However, the function of the TRα2 splicing isoform, which does not bind thyroid hormones, remains unexplored. As no reliable antibodies are available to investigate TRα1 and TRα2 specifically, we introduced small fusion tags into the THRA locus of the male healthy donor iPSC lines BIHi001-B and BIHi005-A by CRISPR/Cas9-mediated genome editing. Consequently, the modified lines express C-terminally tagged TRα1-2xHA or TRα2-3xFLAG. These genome-edited lines facilitate the investigation of isoform-specific actions of TRα in different cell types.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.