芦花提取物超声辅助绿色合成纳米银及抗肿瘤评价

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-04-11 DOI:10.1016/j.ultsonch.2025.107340

Nikita Gandlevskiy , Altevir Rossato Viana , Gabriel Toneto Druzian , Daiana Kaminski Oliveira , Andre Passaglia Schuch , Alessandro Barge , Giancarlo Cravotto , Erico Marlon Moraes Flores

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引用次数: 0

摘要

本研究旨在评价超声辅助合成银纳米粒子（AgNPs）的方法，该方法是基于芦花水提物将Ag+还原为Ag0。此外，还评价了合成的AgNPs对肿瘤细胞的生物活性。考察了Ag+溶液（0.1 mol L-1 Ag+）与石竹提取物的比例、石竹提取物的pH（5、7、9）、含Ag+和石竹提取物的溶液温度、超声类型（槽和探针）、超声频率（槽37、80 kHz，探针20 kHz）、超声振幅和超声时间等参数。为了证实US效应，我们进行了“无声”实验（没有US）。在最佳条件下（US浴，Ag+溶液与R. graveolens L.的比例为1 + 5，v v-1, 80 kHz， 70%振幅，70°C, pH 9, 25 min超声时间），可以得到AgNPs的平均尺寸，PI和zeta电位分别为30 nm， 0.129, - 34.44 mV。为了比较结果，在“沉默”条件下合成的AgNPs的平均尺寸、PI和zeta电位分别为66 nm、0.412和-22.12 mV。与磁性搅拌相比，美国合成的AgNPs更低、更均匀、更稳定。此外，使用US制备的AgNPs的形貌以球形和单分散为主。利用HaCat（角质形成细胞）、L929（成纤维细胞）和B16-F10（黑色素瘤）细胞系对US合成的纳米颗粒的生物活性进行了评价，并通过2,2-二苯基-1-picrylhydrazyl （DPPH）法测定了AgNPs的抗氧化活性。与正常细胞相比，对黑色素瘤细胞系的细胞毒性作用更高（IC50 = 2.12µg mL−1）。DPPH实验结果良好，清除自由基的IC50为234.3µg mL−1。因此，美国技术提出了一种有前途的、可持续的绿色方法，避免了有毒试剂的使用，获得的AgNPs显示出强大的抗癌活性。

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Ultrasound-assisted green synthesis of silver nanoparticles using Ruta graveolens L. Extract and antitumor evaluation

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Ultrasound-assisted green synthesis of silver nanoparticles using Ruta graveolens L. Extract and antitumor evaluation

This study aims to evaluate the ultrasound-assisted synthesis of silver nanoparticles (AgNPs) based on an aqueous extract of Ruta graveolens L. to reduce Ag⁺ to Ag⁰. In addition, the biological activity of the synthesized AgNPs was evaluated against tumor cells. The following parameters were evaluated for the synthesis: proportion between Ag⁺ solution (0.1 mol L^-1 Ag⁺) and R. graveolens L. extract, pH of R. graveolens L. extract (5, 7, and 9), temperature of solution containing Ag⁺ and R. graveolens L. extract, ultrasound (US) type (bath and probes), and the parameters for US as frequency (37, and 80 kHz for bath and 20 kHz for probes), amplitude and time of application of US. In order to confirm the US effect, “silent” experiments (without US) were performed. Using the optimized conditions (US bath, proportion between Ag⁺ solution and R. graveolens L. of 1 + 5, v v^-1, 80 kHz, 70 % amplitude, 70 °C, pH 9, and 25 min of sonication time) it was possible to obtain mean size, PI, and zeta potential of AgNPs of 30 nm, 0.129, −34.44 mV, respectively. For comparison of results, AgNPs synthesized in the “silent” condition presented mean size, PI, and zeta potential of 66 nm, 0.412, and –22.12 mV, respectively. The US synthesized AgNPs were lower, more uniform, and stable when compared with magnetic stirring. In addition, the morphology of AgNPs using US was predominantly spherical and monodisperse. The biological activity using cell lines HaCat (keratinocytes), L929 (fibroblasts), and B16-F10 (melanoma) against nanoparticles synthesized using US was evaluated against the different cell lines and the antioxidant activity of the AgNPs was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. A higher cytotoxic effect on the melanoma cell line (IC₅₀ = 2.12 µg mL⁻¹) compared to normal cells. A good result was found in the DPPH assay, with an IC₅₀ of 234.3 µg mL⁻¹ for free radical scavenging. Therefore, the US technology presents a promising and sustainable green method avoiding the use of toxic reagents and obtained AgNPs showed potent anticancer activity.

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.