基于超亲水性氨基酸多离子液体的大稳定电位检测窗口电化学发光传感器用于大肠癌miRNA-128的检测

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-04-22 DOI:10.1016/j.bios.2025.117502

Yunxue Zhu, Zihui Liang, Peilin Wang, Qiang Ma, Zhiquan Zhang

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引用次数: 0

摘要

电极稳定电位检测窗口（PW）是电化学发光（ECL）研究中经常被忽视的问题。本研究设计并合成了一种氨基酸基多离子液体（AAPIL），以解决电极电位检测窗口窄的问题。在此基础上构建了一种生物传感器，克服了该传感器的局限性，增强了ECL信号。还原钛纳米团簇（R-Ti NCs）作为发光探针，具有强ECL信号。此外，具有高离子电导率的AAPIL可以有效地加速电子转移，促进钛纳米团簇的发光。此外，超亲水性AAPIL具有良好的防污性能。因此，采用基于aapi的生物传感器定量miRNA-128，线性检测范围为1 fM ~ 1 nM，检出限低至0.17 fM。由于其优异的电化学性能，该ECL传感器可成功应用于实际结直肠癌样品的检测。

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An electrochemiluminescence sensor with wide stable potential detection window based on superhydrophilic amino acid polyionic liquid for the detection of miRNA-128 in colorectal cancer

The stable potential detection window (PW) of electrodes in the commonly overlooked issue in electrochemiluminescence (ECL) research. In this study, an amino acid-based polyionic liquid (AAPIL) had been designed and synthesized to solve the problem of narrow potential detection window of the electrode. A biosensor was constructed based on the AAPIL to overcome the limitation and enhance ECL signals. The reduced titanium nanoclusters (R-Ti NCs) were used as luminescent probes with strong ECL signal. Moreover, AAPIL with high ionic conductivity effectively accelerated electron transfer and facilitated the luminescence of titanium nanoclusters. In addition, the superhydrophilicity AAPIL had good antifouling properties. Therefore, the AAPIL-based biosensor was used to quantify miRNA-128 with a linear detection range of 1 fM to 1 nM, while the detection limit was as low as 0.17 fM. Owing to its excellent electrochemical property, this ECL sensor can be successfully applied to the detection of actual colorectal cancer samples.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.