Li Ma , Yaya Liu , Jing Sun , Xiaorong Yang , Yingying He , Tingting Zhang , Jingyu Zhao , Zhiqiang Lu , Xizhong Yan , Xingtao Qie
{"title":"JNK途径调控豌豆蚜虫一氧化氮合成抵抗细菌感染的研究","authors":"Li Ma , Yaya Liu , Jing Sun , Xiaorong Yang , Yingying He , Tingting Zhang , Jingyu Zhao , Zhiqiang Lu , Xizhong Yan , Xingtao Qie","doi":"10.1016/j.ibmb.2025.104315","DOIUrl":null,"url":null,"abstract":"<div><div>Compared to other insects, the pea aphid <em>Acyrthosiphon pisum</em> exhibits limited immune responses, particularly due to the absence of many immune genes, including those encoding antimicrobial peptides and key components of the IMD pathway. Prior studies proved that the conserved signaling, Jun N-terminal kinase (JNK) pathway, plays a critical role in the immune system of the pea aphid, and nitric oxide synthase (NOS) is required for the pea aphid's defense against infections. Herein, using <em>in vitro</em> biochemical assays and <em>in vivo</em> bioassays, we demonstrated that the JNK pathway directly regulates the expression of <em>NOS</em> and that the JNK pathway-NOS-NO signal axis is efficient in defending against bacterial infections. The Toll pathway is instrumental for combating bacterial infections, and NO can activate the Toll pathway. The Toll pathway induced by NO regulates the expressions of ROS metabolism, lysosome, and phagocytosis-related genes. NO was identified as a crucial signaling molecule that facilitates communication between the JNK and Toll pathways.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"180 ","pages":"Article 104315"},"PeriodicalIF":3.2000,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The synthesis of nitric oxide regulated by JNK pathway in the pea aphid to defend against bacterial infection\",\"authors\":\"Li Ma , Yaya Liu , Jing Sun , Xiaorong Yang , Yingying He , Tingting Zhang , Jingyu Zhao , Zhiqiang Lu , Xizhong Yan , Xingtao Qie\",\"doi\":\"10.1016/j.ibmb.2025.104315\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Compared to other insects, the pea aphid <em>Acyrthosiphon pisum</em> exhibits limited immune responses, particularly due to the absence of many immune genes, including those encoding antimicrobial peptides and key components of the IMD pathway. Prior studies proved that the conserved signaling, Jun N-terminal kinase (JNK) pathway, plays a critical role in the immune system of the pea aphid, and nitric oxide synthase (NOS) is required for the pea aphid's defense against infections. Herein, using <em>in vitro</em> biochemical assays and <em>in vivo</em> bioassays, we demonstrated that the JNK pathway directly regulates the expression of <em>NOS</em> and that the JNK pathway-NOS-NO signal axis is efficient in defending against bacterial infections. The Toll pathway is instrumental for combating bacterial infections, and NO can activate the Toll pathway. The Toll pathway induced by NO regulates the expressions of ROS metabolism, lysosome, and phagocytosis-related genes. NO was identified as a crucial signaling molecule that facilitates communication between the JNK and Toll pathways.</div></div>\",\"PeriodicalId\":330,\"journal\":{\"name\":\"Insect Biochemistry and Molecular Biology\",\"volume\":\"180 \",\"pages\":\"Article 104315\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-04-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Insect Biochemistry and Molecular Biology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0965174825000591\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Biochemistry and Molecular Biology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0965174825000591","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
The synthesis of nitric oxide regulated by JNK pathway in the pea aphid to defend against bacterial infection
Compared to other insects, the pea aphid Acyrthosiphon pisum exhibits limited immune responses, particularly due to the absence of many immune genes, including those encoding antimicrobial peptides and key components of the IMD pathway. Prior studies proved that the conserved signaling, Jun N-terminal kinase (JNK) pathway, plays a critical role in the immune system of the pea aphid, and nitric oxide synthase (NOS) is required for the pea aphid's defense against infections. Herein, using in vitro biochemical assays and in vivo bioassays, we demonstrated that the JNK pathway directly regulates the expression of NOS and that the JNK pathway-NOS-NO signal axis is efficient in defending against bacterial infections. The Toll pathway is instrumental for combating bacterial infections, and NO can activate the Toll pathway. The Toll pathway induced by NO regulates the expressions of ROS metabolism, lysosome, and phagocytosis-related genes. NO was identified as a crucial signaling molecule that facilitates communication between the JNK and Toll pathways.
期刊介绍:
This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.