MoS2/MoSe2 Planar Heterostructure Nanoslits for Protein Sequencing

Nanopore sensing technology is reshaping proteomics analysis with simplicity, convenience, and high sensitivity. However, it is now facing challenges of random pore clogging and ultrafast speed when proteins penetrate the nanopores. In this computational study, we propose a nanoslit sensing approach based on two-dimensional (2D) MoS₂/MoSe₂ planar heterostructures. Molecular dynamics (MD) simulations of peptide sequencing under a pulling force and applied electric field are performed. Results show that the peptides are confined within the MoSe₂ domain of the heterostructure, and this confinement effect can be optimized by tailoring the nanostripe length. Besides, the pulling force and current signals can be collected simultaneously. Using the customized geometry, characteristic signals of 20 residues can be detected with excellent discrimination. This study elucidates the sensing mechanism of nanoslit sensors based on planar heterostructures and provides theoretical guidance for the design of devices to control molecular transport during nanopore sequencing.