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利用微型CRISPR系统进行病毒诱导的基因组编辑

IF 15.8 1区 生物学 Q1 PLANT SCIENCES
Nature Plants Pub Date : 2025-04-23 DOI:10.1038/s41477-025-01990-2
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引用次数: 0

摘要

设计烟草摇铃病毒，表达RNA引导的TnpB酶ISYmu1和引导RNA。这种微型CRISPR系统在不需要组织培养或植物转化的情况下，使拟南芥的无转基因生殖系基因组编辑成为可能。
本文章由计算机程序翻译，如有差异，请以英文原文为准。

Virus-induced genome editing using a miniature CRISPR system

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Virus-induced genome editing using a miniature CRISPR system
The tobacco rattle virus was engineered to express the RNA-guided TnpB enzyme ISYmu1 and guide RNA. This miniature CRISPR system enabled transgene-free germline genome editing in Arabidopsis thaliana without the need for tissue culture or plant transformation.
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来源期刊
Nature Plants
Nature Plants PLANT SCIENCES-
CiteScore
25.30
自引率
2.20%
发文量
196
期刊介绍： Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.
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