扩展利什曼原虫的遗传密码：用非天然氨基酸表达膜蛋白的新平台

IF 1.1 Q4 CELL BIOLOGY

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-04-24 DOI:10.1134/S1990747824700399

I. S. Levashov, I. A. Kapranov, D. A. Kornilov, P. K. Kuzmichev, A. V. Mishin, V. I. Borshchevskiy

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引用次数: 0

摘要

非天然氨基酸（UAAs）是研究蛋白质结构和动力学的有力工具。它们在蛋白质中的包含可以帮助揭示其功能和结构特征的重要细节，特别是关于膜蛋白。然而，将UAAs有效地结合到膜蛋白中仍然是一个挑战。在这篇综述中，我们探索利用利什曼原虫（Leishmania tarentolae， LEXSY）作为宿主系统表达膜蛋白，并结合UAAs，旨在为未来的研究和药物发现提供一个通用的平台。

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Expanding the Genetic Code in Leishmania tarentolae: A Novel Platform for Membrane Protein Expression with Unnatural Amino Acids

Unnatural amino acids (UAAs) are powerful tools for investigating protein structure and dynamics. Their inclusion in a protein can help reveal important details about its functions and structural features, especially with respect to membrane proteins. However, efficient incorporation of UAAs into membrane proteins remains a challenge. In this review, we explore using Leishmania tarentolae (LEXSY) as a host system to express membrane proteins with incorporated UAAs, aiming to develop a versatile platform for future research and drug discovery.

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来源期刊

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology CELL BIOLOGY-

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.