I. S. Levashov, I. A. Kapranov, D. A. Kornilov, P. K. Kuzmichev, A. V. Mishin, V. I. Borshchevskiy
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Expanding the Genetic Code in Leishmania tarentolae: A Novel Platform for Membrane Protein Expression with Unnatural Amino Acids
Unnatural amino acids (UAAs) are powerful tools for investigating protein structure and dynamics. Their inclusion in a protein can help reveal important details about its functions and structural features, especially with respect to membrane proteins. However, efficient incorporation of UAAs into membrane proteins remains a challenge. In this review, we explore using Leishmania tarentolae (LEXSY) as a host system to express membrane proteins with incorporated UAAs, aiming to develop a versatile platform for future research and drug discovery.
期刊介绍:
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.