Engineering a versatile nanoscale COF-based multienzyme loading strategy to enhance cancer therapy†

Herein, a simple and efficient in situ assembly approach for immobilizing enzymes within nanoscale COFs under ambient conditions is presented. This approach is versatile and can be applied to immobilize a wide range of enzymes. More importantly, a dual-enzyme-loaded COF is synthesized using this in situ assembly method, followed by coating it with MnO₂ to create GOx&HRP@COF@MnO₂. Of note, GOx&HRP@COF@MnO₂ exhibits remarkable therapeutic efficacy, which can be attributed to the synergistic effect of the Mn²⁺-induced Fenton-like reaction and the cascade enzyme-catalyzed reaction mediated by GOx and HRP. Its significant therapeutic efficacy was validated through both in vitro and in vivo experiments.