北海探险中豪华生物传感器的试验:巴伦支、卡拉和拉普捷夫

IF 1.1 Q4 CELL BIOLOGY
G. V. Volkov, U. S. Novoyatlova, A. A. Utkina, V. I. Krolenko, O. P. Konovalova, S. V. Bazhenov, M. Yu. Kolobov, I. V. Manukhov
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引用次数: 0

摘要

基于大肠杆菌和枯草芽孢杆菌全细胞的细菌生物传感器用于研究生物活性化合物和毒物的毒理学特性,适用于生态学研究。这项工作描述了在北海:巴伦支、卡拉和拉普捷夫的海洋研究考察中豪华生物传感器的试验。通过生物感应细胞中应激促进子的激活来估计海底沉积物和海洋无脊椎动物组织中有毒物质的存在。结果表明,收集的样品在激活氧化应激和SOS反应的能力方面一般不表现出毒理学特征。然而,一些样品能够激活生物传感器细菌细胞中DNA糖基化酶启动子的转录。这一效应在一些海底沉积物样品和沙地拟南蝽的节肢动物组织中得到了证实。和一些片足类动物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Trials of lux-Biosensors in Expeditions to the Northern Seas: Barents, Kara, and Laptev

Bacterial biosensors based on the Escherichia coli and Bacillus subtilis whole cells are used to study toxicological characteristics of biologically active compounds and toxicants, and are applicable for ecological studies. This work describes the trials of lux-biosensors in the marine research expedition in the northern seas: Barents, Kara, and Laptev. The presence of toxicants in samples of bottom sediments and tissues of marine invertebrates was estimated by the activation of stress promoters in biosensor cells. It was shown that the collected samples generally did not show toxicological characteristics in terms of the ability to activate oxidative stress and SOS response. However, several samples were able to activate transcription of the DNA glycosylase promoter in biosensor bacterial cells. This effect was shown for some samples of bottom sediments and arthropod tissues from Mesidothea sabini var. sabini., Pycnogonida and some Amphipoda.

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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
28
期刊介绍: Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology   is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.
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