G. V. Volkov, U. S. Novoyatlova, A. A. Utkina, V. I. Krolenko, O. P. Konovalova, S. V. Bazhenov, M. Yu. Kolobov, I. V. Manukhov
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Trials of lux-Biosensors in Expeditions to the Northern Seas: Barents, Kara, and Laptev
Bacterial biosensors based on the Escherichia coli and Bacillus subtilis whole cells are used to study toxicological characteristics of biologically active compounds and toxicants, and are applicable for ecological studies. This work describes the trials of lux-biosensors in the marine research expedition in the northern seas: Barents, Kara, and Laptev. The presence of toxicants in samples of bottom sediments and tissues of marine invertebrates was estimated by the activation of stress promoters in biosensor cells. It was shown that the collected samples generally did not show toxicological characteristics in terms of the ability to activate oxidative stress and SOS response. However, several samples were able to activate transcription of the DNA glycosylase promoter in biosensor bacterial cells. This effect was shown for some samples of bottom sediments and arthropod tissues from Mesidothea sabini var. sabini., Pycnogonida and some Amphipoda.
期刊介绍:
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.