New applied pharmacological approach/trend from aqueous and sulfated extract from Moringa oleifera Lin on irradiated Ehrlich solid tumor bearing mice

The present study was undertaken to identify the chemical compositions of dried leaves and aqueous extracts from Moringa oleifera leaves (MoL) and investigate the antioxidant and antitumor activities of the crude and sulphated extracts of MoL with or without radiation exposure in Ehrlich solid tumor (EST) bearing female albino mice. For the toxicity test four groups of mice were used (5 mice/gp): control group, crude extract (500mg/kg b.wt orally for 7days), sulfated extract (500mg/kg b.wt orally for 7days), and irradiated group (IRR) [5Gy as fractionated dose 1Gy daily for 5 consecutive days]. For the antitumor activity six groups of mice were used (10 per each): Ehrlich solid tumor group (EST), EST+ crude extract (500mg/kg b.wt), EST+ sulfated extract (500mg/kg b.wt), EST+IRR (500mg/kg b.wt), EST+IRR+ crude extract and finally EST+IRR+ sulfated extract. Results showed that, serum ALT, Albumin, creatinine, Reactive oxygen species(ROS) and Total antioxidant activity (TAC) recorded non-significant changes post treatment with crude extract and sulphated extract groups while IRR group showed a significant elevation in ALT, creatinine and ROS in addition, a decrease in TAC. Moreover, crude extract and sulfated extract alone or combined with irradiation reduced tumor volume and induced apoptosis via increase up regulation of gene expression of caspase-3 and cytochromec in tumor tissue. Histological examination of tumor tissues confirmed the biochemical results. In conclusion, crude extract and sulfated extract have antitumor and radiosenstizing activities against Ehrlich solid tumor via enhancement of apoptotic markers.