dnazyme驱动的Z-scheme g-C3N4/V2C异质结再激活用于细菌活力的光电化学评估

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-04-21 DOI:10.1016/j.bios.2025.117503

Yi Deng , Shuqi Quan , Erkang Tian, Jianwen Dong, Meihong Guo, Juan Li, Hao Yang, Shuangquan Lai

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引用次数: 0

摘要

细菌活力评估对食品和临床生物安全至关重要，因为它揭示了病原体的生存和代谢状态，有助于预测它们持续存在和致病的潜力。在这里，我们提出了一种可见光驱动的光电化学试验（称为DzPEC），设计用于信号检测活致病菌。DzPEC实验采用dnazyme功能化的Z-scheme g-C3N4/V2C生物异质结作为光响应材料，SiO2作为光猝灭剂。DzPEC试验利用DNAzyme靶向分泌到细胞外基质中的代谢性内蛋白RNase H2，可以评估细菌的生存能力。利用具有高光活性的z型异质结，作为细菌模型的肠沙门氏菌（S. enterica） DzPEC检测显示出141 CFU/mL的检出限和低至0.1%的活菌丰度。DzPEC检测与RT-qPCR检测在复杂食品和临床基质中肠链球菌污染有很强的相关性。这些发现突出了DzPEC测定在细菌活力表型和活菌相关生物安全监测方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

DNAzyme-driven Z-scheme g-C3N4/V2C heterojunction reactivation for photoelectrochemical assessment of bacterial viability

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DNAzyme-driven Z-scheme g-C3N4/V2C heterojunction reactivation for photoelectrochemical assessment of bacterial viability

Assessment of bacterial viability is vital for food and clinical biosafety, as it reveals pathogen survival and metabolic state, helping predict their potential to persist and cause illness. Herein, we presented a visible light-driven photoelectrochemical assay (termed DzPEC) designed for signal-on detection of live pathogenic bacteria. The DzPEC assay was fabricated using a DNAzyme-functionalized Z-scheme g-C₃N₄/V₂C bio-heterojunction as a photoresposive material and SiO₂ as a photoquencher. The DzPEC assay, which utilizes DNAzyme to target the metabolic endoprotein RNase H2 that is secreted into the extracellular matrix, allows for bacterial viability assessment. Leveraging the Z-scheme heterojunction with high photoactive performance, the DzPEC assay for Salmonella enterica (S. enterica), used as a bacteria model, exhibited a detection limit of 141 CFU/mL and the ability to detect live bacterial abundnace as low as 0.1 %. The DzPEC assay demonstrated a strong correlation with RT-qPCR in the detection of S. enterica contamination in complex food and clinical matrices. These findings highlighted the potential of the DzPEC assay in bacterial viability phenotyping and live bacteria-associated biosafety monitoring.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.