利用猪特异性msgn1上游区域报告系统监测猪胚胎干细胞近轴中胚层分化

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-04-21 DOI:10.1016/j.scr.2025.103724

Jinsol Jeong , Kwang-Hwan Choi , Dong-Kyung Lee , Yelim Ahn , Chang-Kyu Lee

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引用次数: 0

摘要

以猪特异性MSGN1启动子（pMSGN1）下的EGFP序列为基础，通过电穿孔将基于上游区域的MSGN1报告系统导入猪胚胎干细胞。工程细胞系保持了与亲本细胞系相似的多能性，并且在诱导近轴中胚层时证实了报告活性。pMSGN1-EGFP报告系统是监测近轴中胚层分化过程和分析诱导效率的有价值的工具，为深入了解发育机制提供了依据。

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Pig embryonic stem cells with porcine specific-MSGN1 upstream region-based reporter system for monitoring paraxial mesoderm differentiation

Pig embryonic stem cells were introduced with an MSGN1 upstream region-based reporter system, composed of EGFP sequence under porcine-specific MSGN1 promoter (pMSGN1), through electroporation. The engineered cell line maintained pluripotency similar to that of the parental cell line, and reporter activity was confirmed upon the induction of the paraxial mesoderm. The pMSGN1-EGFP reporter system is a valuable tool for monitoring the differentiation process of the paraxial mesoderm and analyzing induction efficiency, providing insight into developmental mechanisms.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.