厌氧氨氧化颗粒污泥中多糖的特性及其在水凝胶制备中的潜在应用

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-04-22 DOI:10.1016/j.watres.2025.123710

Jie Liu, Yang Liu, Zi Zhang, Yangfan Deng, Guanghao Chen

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引用次数: 0

摘要

能够进行厌氧氨氧化（anammox）或将亚硝酸盐和铵转化为二氮的微生物往往会聚集在一起，在厌氧反应器中形成颗粒状污泥。这种厌氧颗粒污泥具有丰富多样的微生物群落和丰富的聚合物，是多糖的潜在来源。本研究从厌氧颗粒污泥中提取了厌氧多糖（APS），并研究了其与海藻酸盐形成水凝胶的潜力。APS 的产量为 9.91% ± 0.12%。APS 中的三种主要单糖是葡萄糖（60.63% ± 3.45%）、葡萄糖醛酸（13.81% ± 0.31%）和鼠李糖（18.88% ± 0.22%）。通过三种抗氧化试验评估了 APS 的抗氧化潜力，结果表明 APS 浓度在 100 至 500 毫克/升之间时具有显著的抗氧化功效。此外，L929小鼠成纤维细胞在不同的APS浓度（1-50 μg/mL）下都表现出很高的存活率（85%），这表明APS具有良好的生物相容性。将海藻酸盐与 APS 按不同比例（10:0、9:1、8:2、7:3 和 6:4）混合，制备了一系列水凝胶。制备的水凝胶在模拟胃液中的溶胀能力介于 1.4 和 2.0 之间。相反，当水凝胶中海藻酸盐与 APS 的比例为 8:2 时，水凝胶在模拟肠液中的溶胀能力明显增加到 10.37 ± 0.01。此外，还利用 X 射线光电子能谱和傅立叶变换红外光谱分析了水凝胶中的官能团和特定化学键。随后使用牛血清白蛋白（BSA）进行的负载实验表明，海藻酸盐与 APS 的比例为 8:2，对 BSA 的负载效率最高，达到 80.59% ± 1.46%。随着 APS 数量的增加，BSA 在模拟胃液中的释放受到有效抑制，海藻酸与 APS 的比例为 6:4，释放量最低（干态为 0.023%，湿态为 0.11%）。总之，这项研究强调了从厌氧污泥中提取宝贵资源的重要性，并为其在给药领域的潜在应用提供了启示。

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Characterisation of polysaccharide from anammox granular sludge and potential application in hydrogel preparation

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Characterisation of polysaccharide from anammox granular sludge and potential application in hydrogel preparation

Microorganisms capable of anaerobic ammonia oxidation (anammox), or the conversion of nitrite and ammonium to dinitrogen, tend to aggregate and form a granular sludge in anammox reactors. This anammox granular sludge is a potential source of polysaccharides due to its richly diverse microbial community and abundant polymers. In this study, anammox polysaccharide (APS) was extracted from anammox granular sludge, and its potential to form hydrogels with alginate was investigated. The yield of APS was 9.91% ± 0.12%. The three main monosaccharides in APS were glucose (60.63% ± 3.45%), glucuronic acid (13.81% ± 0.31%), and rhamnose (18.88% ± 0.22%). The antioxidant potential of APS was evaluated through three antioxidant assays, which revealed significant antioxidant benefits at APS concentrations between 100 and 500 mg/L. Furthermore, L929 mouse fibroblasts exhibited high survival rates (>85%) under different APS concentrations (1–50 μg/mL), indicating the good biological compatibility of APS. A series of hydrogels were prepared by mixing alginate with APS in different ratios (10:0, 9:1, 8:2, 7:3, and 6:4). The swelling ability of the prepared hydrogels in simulated gastric fluid varied between 1.4 and 2.0. In contrast, the swelling ability increased significantly to 10.37 ± 0.01 in simulated intestinal fluid when the ratio of alginate to APS in the hydrogel was 8:2. X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy were also used to analyse the functional groups and specific chemical bonds in the hydrogels. Subsequent loading experiments using bovine serum albumin (BSA) demonstrated that an alginate:APS ratio of 8:2 exhibited the highest loading efficiency for BSA, reaching 80.59% ± 1.46%. As the quantity of APS was increased, the release of BSA into simulated gastric fluid was effectively inhibited, with an alginate:APS ratio of 6:4 resulting in the lowest release amount (0.023% in dry state, 0.11% in wet state). Overall, this study highlights the derivation of a valuable resource from anammox sludge and offers insights into its potential applications in drug delivery.

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.