Bacterial community analysis of recreational beach waters reveals human fecal contamination and pathogenicity across varying field conditions

Assessing human fecal contamination of recreational beach waters is a public health challenge owing to multiple sources and environmental conditions affecting indicator, pathogen, and source marker relationships. Human fecal-associated markers, such as HF183, accurately indicate human waste, but reliance on individual markers risks false negative diagnoses, owing to marker source loading variations and attenuation. Bacterial community analysis is a data-dense approach that could improve the accuracy of human waste detection, but relatedness to actual human health hazards under complex coastal water conditions is unproven. Here, two Malibu and one Pacific Palisades, CA recreational beaches differing in urbanization and sewerage were studied over two years across varying seasonal rainfall, and beach berm, conditions. Fecal indicator bacteria (FIB), HF183, and human norovirus concentrations were quantified and related to bacterial community 16S rRNA gene sequences which were further analyzed for putative human pathogens and sewage proportions. All lower watersheds harbored human fecal sources, and surf zone contamination was enhanced by rainfall runoff with berm breaching, which intensified with urbanization. Notably, for sequenced surf zone waters sampled across all weather conditions (n = 36), sequence-based putative pathogen proportions correlated with qPCR HF183 concentrations (w/ and w/o PMA treatment; p = 0.0) and with human norovirus G1concentrations (p = 0.04) which also correlated with HF183 sequence abundance in bacterial communities (p = 0.02). Although human fecal sources appeared to vary seasonally and a range of physical conditions influenced surf zone fecal contamination, human fecal contamination and associated health hazard were reliably evidenced by bacterial community analysis in this study.