中国细藻ACCase W2027C突变的快速检测使用RAA-CRISPR/Cas12a：一种敏感和可视化的除草剂抗性诊断工具

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2025-04-23 DOI:10.1002/ps.8840

Shu Liu, Ziheng Cao, Jiahao Lun, Chao Zhang, Lang Pan

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引用次数: 0

摘要

长期施用抗accase除草剂，如氯氟草酰丁酯，导致稻田中广泛出现抗除草剂的中国细藻种群。在ACCase的众多靶位耐药（TSR）突变中，Trp-2027-Cys （W2027C）突变是羊草中最常见的突变之一。随着抗除草剂羊草的日益流行，迫切需要快速准确的诊断工具来检测抗性突变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Rapid detection of ACCase W2027C mutation in Leptochloa chinensis (L.) Nees using RAA-CRISPR/Cas12a: a sensitive and visual tool for herbicide resistance diagnosis

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Rapid detection of ACCase W2027C mutation in Leptochloa chinensis (L.) Nees using RAA-CRISPR/Cas12a: a sensitive and visual tool for herbicide resistance diagnosis

Prolonged application of ACCase-inhibiting herbicides, such as cyhalofop-butyl, has resulted in the widespread emergence of herbicide-resistant Leptochloa chinensis populations in paddy fields. Among the numerous target-site resistance (TSR) mutations in ACCase, the Trp-2027-Cys (W2027C) mutation is one of the most prevalent in L. chinensis. The increasing prevalence of herbicide-resistant L. chinensis highlights the critical need for rapid and precise diagnostic tools to detect resistance mutations.

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.