Lisi Wang , Xiaoqing Li , Baoli Wang , Fan Shi , Brij Mohan , Mansoor Ahmad , Wei Sun
{"title":"获得灵敏度:一种基于辣根过氧化物酶和MoS2@black磷烯的草甘膦检测电化学生物传感器","authors":"Lisi Wang , Xiaoqing Li , Baoli Wang , Fan Shi , Brij Mohan , Mansoor Ahmad , Wei Sun","doi":"10.1016/j.bioelechem.2025.108991","DOIUrl":null,"url":null,"abstract":"<div><div>Glyphosate (GLY) is one of the most widely used herbicides, and the presence of its residues in food samples poses a threat to human health. Developing a monitoring system could help address food safety concerns. This study presents an innovative electrochemical sensing platform to detect GLY, which employs molybdenum disulfide (MoS<sub>2</sub>) and black phosphorene (BP) nanocomposites (MoS<sub>2</sub>@BP) with horseradish peroxidase (HRP) for the working electrode modification. The MoS<sub>2</sub>@BP nanocomposite is synthesized using a hydrothermal method, and its characteristics are investigated through different methods including scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction methods. The presence of BP enhances electrical conductivity and increases specific surface area, while MoS<sub>2</sub> improves the electrochemical properties of the composites and provides a protective effect on BP. The modification of MoS₂@BP on the electrode surface helps to accelerate the direct electron transfer of HRP with enhanced electrochemical responses. Furthermore, the incorporation of amino acid residues from HRP significantly enhances the recognition of GLY, thereby improving the selectivity and sensitivity of this electrochemical sensor. The sensor operates effectively within a linear concentration range from 0.118 nmol/L to 20.65 nmol/L, with a low detection limit of 0.0393 nmol/L (3σ). Furthermore, the sensor is successfully applied to detect GLY in real cornmeal samples with satisfactory results, demonstrating its potential applications in food safety monitoring.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108991"},"PeriodicalIF":4.8000,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Garnering sensitivity: A horseradish peroxidase and MoS2@black phosphorene based electrochemical biosensor for glyphosate detection\",\"authors\":\"Lisi Wang , Xiaoqing Li , Baoli Wang , Fan Shi , Brij Mohan , Mansoor Ahmad , Wei Sun\",\"doi\":\"10.1016/j.bioelechem.2025.108991\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Glyphosate (GLY) is one of the most widely used herbicides, and the presence of its residues in food samples poses a threat to human health. Developing a monitoring system could help address food safety concerns. This study presents an innovative electrochemical sensing platform to detect GLY, which employs molybdenum disulfide (MoS<sub>2</sub>) and black phosphorene (BP) nanocomposites (MoS<sub>2</sub>@BP) with horseradish peroxidase (HRP) for the working electrode modification. The MoS<sub>2</sub>@BP nanocomposite is synthesized using a hydrothermal method, and its characteristics are investigated through different methods including scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction methods. The presence of BP enhances electrical conductivity and increases specific surface area, while MoS<sub>2</sub> improves the electrochemical properties of the composites and provides a protective effect on BP. The modification of MoS₂@BP on the electrode surface helps to accelerate the direct electron transfer of HRP with enhanced electrochemical responses. Furthermore, the incorporation of amino acid residues from HRP significantly enhances the recognition of GLY, thereby improving the selectivity and sensitivity of this electrochemical sensor. The sensor operates effectively within a linear concentration range from 0.118 nmol/L to 20.65 nmol/L, with a low detection limit of 0.0393 nmol/L (3σ). Furthermore, the sensor is successfully applied to detect GLY in real cornmeal samples with satisfactory results, demonstrating its potential applications in food safety monitoring.</div></div>\",\"PeriodicalId\":252,\"journal\":{\"name\":\"Bioelectrochemistry\",\"volume\":\"165 \",\"pages\":\"Article 108991\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2025-04-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioelectrochemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567539425000945\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioelectrochemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567539425000945","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Garnering sensitivity: A horseradish peroxidase and MoS2@black phosphorene based electrochemical biosensor for glyphosate detection
Glyphosate (GLY) is one of the most widely used herbicides, and the presence of its residues in food samples poses a threat to human health. Developing a monitoring system could help address food safety concerns. This study presents an innovative electrochemical sensing platform to detect GLY, which employs molybdenum disulfide (MoS2) and black phosphorene (BP) nanocomposites (MoS2@BP) with horseradish peroxidase (HRP) for the working electrode modification. The MoS2@BP nanocomposite is synthesized using a hydrothermal method, and its characteristics are investigated through different methods including scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction methods. The presence of BP enhances electrical conductivity and increases specific surface area, while MoS2 improves the electrochemical properties of the composites and provides a protective effect on BP. The modification of MoS₂@BP on the electrode surface helps to accelerate the direct electron transfer of HRP with enhanced electrochemical responses. Furthermore, the incorporation of amino acid residues from HRP significantly enhances the recognition of GLY, thereby improving the selectivity and sensitivity of this electrochemical sensor. The sensor operates effectively within a linear concentration range from 0.118 nmol/L to 20.65 nmol/L, with a low detection limit of 0.0393 nmol/L (3σ). Furthermore, the sensor is successfully applied to detect GLY in real cornmeal samples with satisfactory results, demonstrating its potential applications in food safety monitoring.
期刊介绍:
An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry
Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of:
• Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction.
• Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms)
• Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes)
• Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion)
• Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair).
• Organization and use of arrays in-vitro and in-vivo, including as part of feedback control.
• Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.