研究碱处理对食糖komagataeibacterium BC-G1合成细菌纤维素理化特性的影响

The Microbe Pub Date : 2025-04-19 DOI:10.1016/j.microb.2025.100340

Samriddh Srivastava, Garima Mathur

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引用次数: 0

摘要

细菌纤维素（BC）由于其多用途的特性和广泛的工业应用而引起了全球的广泛关注。然而，大规模生产BC面临着挑战，特别是在净化过程中，有效去除细菌生物量和培养基是至关重要的。传统的碱处理是常用的，但不同碱浓度对BC物理化学性质的影响尚未得到充分研究。因此，本研究研究了不同浓度的氢氧化钠（NaOH）（0-8 % w/v）对从腐烂葡萄中分离出来的Komagataeibacter saccharivorans BC- g1产生的BC的溶解度、结构和化学特性的影响。采用HS、M1、M2和M3四种培养基在静态条件下生产BC， 5天后M3培养基产量最高，为104.42 ± 1.48 mg/100 mL， M2培养基产量为19.2 ± 2.10 mg/100 mL。采用FTIR、XRD、DSC和SEM对其理化性质进行了表征。FTIR分析显示，在HS、M1、M2和M3培养基中，结晶度指数的变化范围分别为1.08（标准BC）至0.98、1.01、0.97和0.91。DSC分析显示结晶温度（Tc）为83℃~ 112℃，熔融温度（Tm）为293℃~ 445℃，玻璃化转变温度（Tg）为28.8℃~ 64.5℃。结果表明，碱处理破坏了纤维素结构内的氢键，导致氢键模式和空间排列发生变化。高浓度NaOH诱导了纤维素I向纤维素II的转变，突出了碱浓度在决定BC性质中的重要性。

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Investigating the effect of alkali treatment on physicochemical characteristics of bacterial cellulose synthesized by Komagataeibacter saccharivorans BC-G1

Bacterial Cellulose (BC) has gained significant global interest due to its versatile properties and wide range of industrial applications. However, large-scale BC production faces challenges, particularly in the purification process, where effective removal of bacterial biomass and culture media is critical. Traditional alkali treatments are commonly used, yet the effects of varying alkali concentrations on BC's physicochemical properties are underexplored. Therefore, this study investigates the impact of different sodium hydroxide (NaOH) concentrations (0–8 % w/v) on the solubility, structural, and chemical characteristics of BC produced by Komagataeibacter saccharivorans BC-G1, isolated from rotten grapes. BC was produced under static conditions using four different culture media (HS, M1, M2, and M3), with the highest yield of 104.42 ± 1.48 mg/100 mL in M3 medium after 5 days, compared to 19.2 ± 2.10 mg/100 mL in M2. Physicochemical properties were characterized using FTIR, XRD, DSC, and SEM. FTIR analysis revealed variations in the crystallinity index ranged from 1.08 (standard BC) to 0.98, 1.01, 0.97, and 0.91 in HS, M1, M2, and M3 media, respectively. DSC analysis exhibited crystallization temperatures (Tc) from 83 °C to 112 °C, melting temperatures (Tm) from 293 °C to 445 °C, and glass transition temperatures (Tg) from 28.8 °C to 64.5 °C. Results revealed that alkali treatment disrupted hydrogen bonding within the cellulose structure, leading to changes in bonding patterns and spatial arrangement. High NaOH concentrations induced a transition from cellulose I to cellulose II, highlighting the importance of alkali concentration in determining BC properties.

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The Microbe

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