{"title":"盐桥介导的富含精氨酸的凝聚体向囊泡的转化","authors":"Hanjin Seo, Hyun Su Lee, Hyomin Lee","doi":"10.1021/acsnano.5c02235","DOIUrl":null,"url":null,"abstract":"Polypeptide-based liquid–liquid phase separation (LLPS) has received considerable attention as it governs the formation of membraneless organelles in cells. However, the detailed mechanistic understanding of how one of the most prevalent cationic amino acids in proteins, arginine, interacts with various biomolecules to induce phase separation and undergo morphogenesis remains to be resolved. Herein, we report the phase separation behavior and transformation of arginine-rich coacervates into vesicular structures upon introducing polyphosphates. Transformation into vesicles was shown to occur independent of the initial anionic counterparts and was driven by salt-bridge interactions between guanidinium groups of arginine residues and phosphates. We also investigate the role of intermolecular forces and ionic effects on the morphological transformation and further exploit their potential in the assembly of artificial tissue-like constructs. Overall, our findings underpin a unifying principle for vesicle transformation from arginine-rich coacervates and their potency for reconstituting hierarchical biological microcompartments.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"41 1","pages":""},"PeriodicalIF":16.0000,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Salt-Bridge-Mediated Coacervate-to-Vesicle Transformation in Arginine-Rich Coacervates\",\"authors\":\"Hanjin Seo, Hyun Su Lee, Hyomin Lee\",\"doi\":\"10.1021/acsnano.5c02235\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Polypeptide-based liquid–liquid phase separation (LLPS) has received considerable attention as it governs the formation of membraneless organelles in cells. However, the detailed mechanistic understanding of how one of the most prevalent cationic amino acids in proteins, arginine, interacts with various biomolecules to induce phase separation and undergo morphogenesis remains to be resolved. Herein, we report the phase separation behavior and transformation of arginine-rich coacervates into vesicular structures upon introducing polyphosphates. Transformation into vesicles was shown to occur independent of the initial anionic counterparts and was driven by salt-bridge interactions between guanidinium groups of arginine residues and phosphates. We also investigate the role of intermolecular forces and ionic effects on the morphological transformation and further exploit their potential in the assembly of artificial tissue-like constructs. Overall, our findings underpin a unifying principle for vesicle transformation from arginine-rich coacervates and their potency for reconstituting hierarchical biological microcompartments.\",\"PeriodicalId\":21,\"journal\":{\"name\":\"ACS Nano\",\"volume\":\"41 1\",\"pages\":\"\"},\"PeriodicalIF\":16.0000,\"publicationDate\":\"2025-04-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Nano\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://doi.org/10.1021/acsnano.5c02235\",\"RegionNum\":1,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Nano","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1021/acsnano.5c02235","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Salt-Bridge-Mediated Coacervate-to-Vesicle Transformation in Arginine-Rich Coacervates
Polypeptide-based liquid–liquid phase separation (LLPS) has received considerable attention as it governs the formation of membraneless organelles in cells. However, the detailed mechanistic understanding of how one of the most prevalent cationic amino acids in proteins, arginine, interacts with various biomolecules to induce phase separation and undergo morphogenesis remains to be resolved. Herein, we report the phase separation behavior and transformation of arginine-rich coacervates into vesicular structures upon introducing polyphosphates. Transformation into vesicles was shown to occur independent of the initial anionic counterparts and was driven by salt-bridge interactions between guanidinium groups of arginine residues and phosphates. We also investigate the role of intermolecular forces and ionic effects on the morphological transformation and further exploit their potential in the assembly of artificial tissue-like constructs. Overall, our findings underpin a unifying principle for vesicle transformation from arginine-rich coacervates and their potency for reconstituting hierarchical biological microcompartments.
期刊介绍:
ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.