一种用金（银）纳米粒子装饰的纤维素纸，用于基于sers的免疫测定

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-04-21 DOI:10.1016/j.talanta.2025.128170

Tao Ma , Xiaoqiang Dong , Iris Liang , Jiuzhou Chen , Yi Tao , Junling Chen , Dandan Xu , Feng Liang

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引用次数: 0

摘要

基于表面增强拉曼散射（SERS）的免疫分析为生物标志物的早期诊断提供了一种前所未有的方法。然而，可靠性方面的挑战往往阻碍了它们的广泛应用。本研究介绍了一种新的方法，利用在原位制备的纤维素纸上装饰金和金银纳米粒子（Au(-Ag) CP）来构建高灵敏度和稳定的纸基SERS底物。在此基础上，我们开发了强大的SERS免疫底物和免疫探针，实现了多功能免疫分析平台。该平台在检测疾病标志物方面表现出优异的性能。使用金纳米颗粒探针与Au CP结合，对COVID-19抗原的检出限（LOD）为29.71 fg/mL，而Au- ag CP与Au核ag壳（Au@Ag）探针结合，对心脏肌钙蛋白I （cTnI）的检出限为10.79 fg/mL。两种检测模式都具有从10−13到10−8 g/mL的宽动态范围和持续的稳定性，在5周的储存后保持相当的LOD。本文提出的基于纸张的SERS免疫分析平台有望实现快速、超灵敏的生物标志物检测，为推进诊断技术提供一种变革性工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A cellulose paper decorated with gold(-silver) nanoparticles for SERS-based immunoassays

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A cellulose paper decorated with gold(-silver) nanoparticles for SERS-based immunoassays

Surface-enhanced Raman scattering (SERS)-based immunoassays offer an unprecedented method for the early diagnosis of biomarkers. However, challenges in reliability often hinder their broader applications. This study introduces a novel approach employing in situ-prepared cellulose paper decorated with gold and gold-silver nanoparticles (Au(-Ag) CP) to construct a highly sensitive and stable paper-based SERS substrate. Building on this, we developed robust SERS immunosubstrates and immunoprobes, enabling a versatile immunoassay platform. This platform demonstrated exceptional performance in detecting disease markers. Using gold nanoparticles probe with Au CP, a limit of detection (LOD) of 29.71 fg/mL was achieved for the COVID-19 antigen, while the Au–Ag CP coupled with the Au-core Ag-shell (Au@Ag) probes achieved an LOD of 10.79 fg/mL for cardiac troponin I (cTnI). Both detection modes featured a broad dynamic range from 10⁻¹³ to 10⁻⁸ g/mL and sustained stability, retaining comparable LOD after 5 weeks of storage. The paper-based SERS immunoassay platform presented here holds promise for rapid, ultra-sensitive biomarker detection, offering a transformative tool for advancing diagnostic technologies.

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.