大鼠颞下颌关节盘组织及其衍生细胞基因表达谱分析

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-04-21 DOI:10.1016/j.jobcr.2025.03.018

Haruna Kashio , Rie Takai , Ayuko Takada , Yuya Nakao , Nattakarn Hosiriluck , Masahiro Iijima , Yoshihiro Abiko , Itaru Mizoguchi , Toshiya Arakawa

{"title":"大鼠颞下颌关节盘组织及其衍生细胞基因表达谱分析","authors":"Haruna Kashio , Rie Takai , Ayuko Takada , Yuya Nakao , Nattakarn Hosiriluck , Masahiro Iijima , Yoshihiro Abiko , Itaru Mizoguchi , Toshiya Arakawa","doi":"10.1016/j.jobcr.2025.03.018","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>The temporomandibular joint (TMJ) disc is composed of a fibrocartilaginous connective tissue. Its dysfunction, resulting from excessive jaw movement, can lead to TMJ disorders. In this study, we aimed to investigate the crucial molecular information regarding the extracellular matrix (ECM), which would be necessary for treating such disorders through TMJ disc regeneration. To achieve this, we compared the gene profiles of TMJ disc tissues, their derived cells, and the periodontal ligament (PDL) in our previous study.</div></div><div><h3>Methods</h3><div>TMJ discs were isolated from male Wistar rats. Cells derived from the TMJ discs were cultured, and mRNA extracted from the disc tissues and derived cells was analyzed for gene profiling via microarray hybridization. Additionally, we compared the ECM expression between the TMJ disc and the PDL.</div></div><div><h3>Results</h3><div>Collagen (types I, II, III, and VI) and proteoglycans (biglycan and fibromodulin) were highly expressed in the TMJ discs. Significant reduction in decorin, fibromodulin and COL2 were observed in the TMJ-derived cells than in the tissue. Type VI collagen was the third most highly expressed in both the TMJ disc and PDL tissues, following types I and III.</div></div><div><h3>Conclusions</h3><div>Collagen types VI and II were prominently expressed, followed by collagen types I and III, in TMJ disc tissues, reflecting the unique functions of the disc. Type VI collagen was highly expressed in both TMJ disc and PDL tissues. Overall, type VI collagen might be a key molecule for TMJ disc regeneration, ensuring elasticity and cushioning, and could provide new insights for TMJ regeneration.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 4","pages":"Pages 712-717"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The profiling and analysis of gene expression in rat temporomandibular joint disc tissue and its derived cells\",\"authors\":\"Haruna Kashio , Rie Takai , Ayuko Takada , Yuya Nakao , Nattakarn Hosiriluck , Masahiro Iijima , Yoshihiro Abiko , Itaru Mizoguchi , Toshiya Arakawa\",\"doi\":\"10.1016/j.jobcr.2025.03.018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div>The temporomandibular joint (TMJ) disc is composed of a fibrocartilaginous connective tissue. Its dysfunction, resulting from excessive jaw movement, can lead to TMJ disorders. In this study, we aimed to investigate the crucial molecular information regarding the extracellular matrix (ECM), which would be necessary for treating such disorders through TMJ disc regeneration. To achieve this, we compared the gene profiles of TMJ disc tissues, their derived cells, and the periodontal ligament (PDL) in our previous study.</div></div><div><h3>Methods</h3><div>TMJ discs were isolated from male Wistar rats. Cells derived from the TMJ discs were cultured, and mRNA extracted from the disc tissues and derived cells was analyzed for gene profiling via microarray hybridization. Additionally, we compared the ECM expression between the TMJ disc and the PDL.</div></div><div><h3>Results</h3><div>Collagen (types I, II, III, and VI) and proteoglycans (biglycan and fibromodulin) were highly expressed in the TMJ discs. Significant reduction in decorin, fibromodulin and COL2 were observed in the TMJ-derived cells than in the tissue. Type VI collagen was the third most highly expressed in both the TMJ disc and PDL tissues, following types I and III.</div></div><div><h3>Conclusions</h3><div>Collagen types VI and II were prominently expressed, followed by collagen types I and III, in TMJ disc tissues, reflecting the unique functions of the disc. Type VI collagen was highly expressed in both TMJ disc and PDL tissues. Overall, type VI collagen might be a key molecule for TMJ disc regeneration, ensuring elasticity and cushioning, and could provide new insights for TMJ regeneration.</div></div>\",\"PeriodicalId\":16609,\"journal\":{\"name\":\"Journal of oral biology and craniofacial research\",\"volume\":\"15 4\",\"pages\":\"Pages 712-717\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-04-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of oral biology and craniofacial research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212426825000818\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral biology and craniofacial research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212426825000818","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

摘要

目的颞下颌关节盘由纤维软骨结缔组织构成。它的功能障碍，由于过度的下颌运动，可导致颞下颌关节紊乱。在这项研究中，我们旨在研究有关细胞外基质（ECM）的关键分子信息，这将是通过TMJ椎间盘再生治疗此类疾病所必需的。为了实现这一目标，我们比较了TMJ椎间盘组织及其衍生细胞和牙周韧带（PDL）的基因谱。方法从雄性Wistar大鼠中分离stmj椎间盘。培养来自TMJ椎间盘的细胞，通过微阵列杂交分析从椎间盘组织和衍生细胞中提取的mRNA进行基因谱分析。此外，我们比较了TMJ椎间盘和PDL之间ECM的表达。结果胶原蛋白（I、II、III和VI型）和蛋白聚糖（高聚糖和纤维调节蛋白）在TMJ椎间盘中高表达。tmj源性细胞中decorin、fibromodulin和COL2的表达明显低于组织。在TMJ椎间盘和PDL组织中，VI型胶原是第三高表达的，仅次于I型和III型。结论在TMJ椎间盘组织中，VI型和II型胶原蛋白表达显著，其次是I型和III型胶原蛋白，反映了椎间盘的独特功能。VI型胶原在TMJ椎间盘和PDL组织中均有高表达。总之，VI型胶原蛋白可能是保证TMJ椎间盘再生弹性和缓冲的关键分子，可能为TMJ再生提供新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The profiling and analysis of gene expression in rat temporomandibular joint disc tissue and its derived cells

查看原文本刊更多论文

The profiling and analysis of gene expression in rat temporomandibular joint disc tissue and its derived cells

Objective

The temporomandibular joint (TMJ) disc is composed of a fibrocartilaginous connective tissue. Its dysfunction, resulting from excessive jaw movement, can lead to TMJ disorders. In this study, we aimed to investigate the crucial molecular information regarding the extracellular matrix (ECM), which would be necessary for treating such disorders through TMJ disc regeneration. To achieve this, we compared the gene profiles of TMJ disc tissues, their derived cells, and the periodontal ligament (PDL) in our previous study.

Methods

TMJ discs were isolated from male Wistar rats. Cells derived from the TMJ discs were cultured, and mRNA extracted from the disc tissues and derived cells was analyzed for gene profiling via microarray hybridization. Additionally, we compared the ECM expression between the TMJ disc and the PDL.

Results

Collagen (types I, II, III, and VI) and proteoglycans (biglycan and fibromodulin) were highly expressed in the TMJ discs. Significant reduction in decorin, fibromodulin and COL2 were observed in the TMJ-derived cells than in the tissue. Type VI collagen was the third most highly expressed in both the TMJ disc and PDL tissues, following types I and III.

Conclusions

Collagen types VI and II were prominently expressed, followed by collagen types I and III, in TMJ disc tissues, reflecting the unique functions of the disc. Type VI collagen was highly expressed in both TMJ disc and PDL tissues. Overall, type VI collagen might be a key molecule for TMJ disc regeneration, ensuring elasticity and cushioning, and could provide new insights for TMJ regeneration.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.