通过调节共价相互作用调节共价有机框架中Cyt - c的活性和稳定性

IF 4.9 2区化学 Q2 CHEMISTRY, PHYSICAL

Colloids and Surfaces A: Physicochemical and Engineering Aspects Pub Date : 2025-04-17 DOI:10.1016/j.colsurfa.2025.136973

Yu-Xin Ma , Jin-Zhou Liu , Xi Zhang , Qiu-Lin Wen , Rong Sheng Li , Jian Ling , Shu Jun Zhen , Qiue Cao , Cheng Zhi Huang

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引用次数: 0

摘要

调控微环境以优化酶的催化性能和稳定性是酶固定化研究面临的挑战。共价有机框架（COFs）由于具有良好的可调孔环境，为酶固定化提供了一个很有前景的平台。本文在室温下合成了孔洞中不同羟基含量的COF ([OH]x%-TD-COF)，并通过共价相互作用将细胞色素c （Cyt c）固定在不同锚定点的COF中。通过调节共价相互作用，可以显著提高酶的稳定性和活性。COF对酶活性的增强使H2O2含量的定量检测更加快速方便。COF通道的可调节性带来的共价相互作用的可调性为酶固定化提供了新的研究方向。

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Modulating the activity and stability of Cyt c in a covalent organic framework by regulating the covalent interactions

Controllable regulation of microenvironment to optimize enzyme catalytic performance and stability is a challenge for enzyme immobilization. Covalent organic frameworks (COFs) provide a promising platform for enzyme immobilization due to their finely tunable pore environment. In this paper, COF with different hydroxyl content in the pore ([OH]_x%-TD-COF) were synthesized at room temperature, and cytochrome c (Cyt c) was immobilized in the COF with different anchoring points through covalent interaction. By adjusting the covalent interaction, the stability and activity of the enzyme can be significantly improved. The enhancement of enzyme activity by COF enabled rapid and convenient quantitative detection of H₂O₂ content. The adjustability of the covalent interaction brought by the adjustability of the COF channel provides a new research direction for enzyme immobilization.

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来源期刊

Colloids and Surfaces A: Physicochemical and Engineering Aspects 化学-物理化学

CiteScore

8.70

自引率

9.60%

发文量

2421

审稿时长

56 days

期刊介绍： Colloids and Surfaces A: Physicochemical and Engineering Aspects is an international journal devoted to the science underlying applications of colloids and interfacial phenomena. The journal aims at publishing high quality research papers featuring new materials or new insights into the role of colloid and interface science in (for example) food, energy, minerals processing, pharmaceuticals or the environment.