在硼限制条件下，肌球蛋白XI需要通过维持硼酸通道AtNIP5;1的内吞和极性定位来进行硼转运

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-04-17 DOI:10.1016/j.plaphy.2025.109938

Haiyang Liu , Keita Muro , Riku Chishima , Junpei Takano , Motoki Tominaga

{"title":"在硼限制条件下，肌球蛋白XI需要通过维持硼酸通道AtNIP5;1的内吞和极性定位来进行硼转运","authors":"Haiyang Liu , Keita Muro , Riku Chishima , Junpei Takano , Motoki Tominaga","doi":"10.1016/j.plaphy.2025.109938","DOIUrl":null,"url":null,"abstract":"<div><div>Myosin XI plays a major role in cytoplasmic streaming and is essential for intracellular transport. Here, we investigated the physiological roles of myosin XI in nutrient transport using double (<em>2ko</em>) and triple (<em>3ko</em>) myosin XI knockout mutants of <em>Arabidopsis thaliana</em>. The results revealed that the mutants exhibited more severe boron deficiency phenotypes under boron-limiting conditions, and the boron concentration in the aerial parts of mutant plants was lower than that in the wild-type. Microscopic analysis demonstrated a reduction in general endocytosis and abolishment of NIP5; 1's polar localization in <em>2ko</em> and <em>3ko</em> plants. Overall, these results indicate that myosin XI is necessary for proper boron transport via the maintenance of the endocytic pathway and NIP5; 1's polar localization.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109938"},"PeriodicalIF":6.1000,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Myosin XI is required for boron transport under boron limitation via maintenance of endocytosis and polar localization of the boric acid channel AtNIP5;1\",\"authors\":\"Haiyang Liu , Keita Muro , Riku Chishima , Junpei Takano , Motoki Tominaga\",\"doi\":\"10.1016/j.plaphy.2025.109938\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Myosin XI plays a major role in cytoplasmic streaming and is essential for intracellular transport. Here, we investigated the physiological roles of myosin XI in nutrient transport using double (<em>2ko</em>) and triple (<em>3ko</em>) myosin XI knockout mutants of <em>Arabidopsis thaliana</em>. The results revealed that the mutants exhibited more severe boron deficiency phenotypes under boron-limiting conditions, and the boron concentration in the aerial parts of mutant plants was lower than that in the wild-type. Microscopic analysis demonstrated a reduction in general endocytosis and abolishment of NIP5; 1's polar localization in <em>2ko</em> and <em>3ko</em> plants. Overall, these results indicate that myosin XI is necessary for proper boron transport via the maintenance of the endocytic pathway and NIP5; 1's polar localization.</div></div>\",\"PeriodicalId\":20234,\"journal\":{\"name\":\"Plant Physiology and Biochemistry\",\"volume\":\"224 \",\"pages\":\"Article 109938\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2025-04-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology and Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0981942825004668\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942825004668","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

肌球蛋白 XI 在细胞质流中发挥着重要作用，对细胞内运输至关重要。在此，我们利用拟南芥双（2ko）和三（3ko）肌球蛋白 XI 基因敲除突变体研究了肌球蛋白 XI 在营养物质运输中的生理作用。结果发现，在硼限制条件下，突变体表现出更严重的缺硼表型，突变体植株气生部分的硼浓度低于野生型。显微镜分析表明，2ko 和 3ko 植株的一般内吞作用减弱，NIP5; 1 的极性定位消失。总之，这些结果表明，肌球蛋白 XI 是通过维持内吞途径和 NIP5; 1 的极性定位来实现正常硼运输的必要条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Myosin XI is required for boron transport under boron limitation via maintenance of endocytosis and polar localization of the boric acid channel AtNIP5;1

Myosin XI plays a major role in cytoplasmic streaming and is essential for intracellular transport. Here, we investigated the physiological roles of myosin XI in nutrient transport using double (2ko) and triple (3ko) myosin XI knockout mutants of Arabidopsis thaliana. The results revealed that the mutants exhibited more severe boron deficiency phenotypes under boron-limiting conditions, and the boron concentration in the aerial parts of mutant plants was lower than that in the wild-type. Microscopic analysis demonstrated a reduction in general endocytosis and abolishment of NIP5; 1's polar localization in 2ko and 3ko plants. Overall, these results indicate that myosin XI is necessary for proper boron transport via the maintenance of the endocytic pathway and NIP5; 1's polar localization.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.