使用树状石墨烯的便携式无线恒电位传感器，用于超灵敏、实时检测基因组DNA中的5hmC

IF 16 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

ACS Nano Pub Date : 2025-04-20 DOI:10.1021/acsnano.4c18646

Habibulla Imran, Sumin Lim, Asrar Alam, Jungeun An, Myunggon Ko, Sooman Lim

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引用次数: 0

摘要

基因组5-羟甲基胞嘧啶（5hmC）的异常改变是5-甲基胞嘧啶（5mC）被10 - 11易位（TET）酶氧化的产物，通常与癌症有关。快速和精确的5hmC定量是至关重要的，因为它是诊断，病理生理学和治疗的关键生物标志物。在这里，我们提出了一种便携式无线恒电位器，用于实时、超灵敏的5hmC-DNA传感，该传感器基于树状石墨烯（teG）修饰的丝网印刷微电极。铅笔石墨的一锅电化学剥离使teG的合成具有高导电性、优异的电化学导电性、低表面粗糙度和高5hmC-DNA吸附性，超过了铅笔石墨（pG）和氧化石墨烯（GO）。teg修饰的金电极具有出色的灵敏度（6.15 × 10-6 mM-1 cm-2）、选择性和重复性，对5hmC-DNA具有12.6 fM的超低检测限。通过量化各种生物标本基因组DNA中的5hmC水平，验证了该传感器的性能，包括TET功能改变的小鼠原代组织、小鼠肝细胞癌和人类前列腺癌细胞系。为了提高实用性，研究人员开发了一种柔性的桑纸丝网印刷微电极，并将其与Arduino Nano 33 IoT供电的便携式无线电位器集成在一起。基于开路电位（OCP）的检测可以实现无标签、实时监测，并通过无线数据传输到Android移动应用程序，成功区分癌细胞和非癌细胞之间的5hmC水平。这些发现突出了teG的高表面积，优越的电荷传输和可扩展性，将其定位为下一代生物传感的有前途的平台。开发的传感器为5hmC定量提供了一种快速、经济、高灵敏度的工具，对早期癌症诊断和治疗具有重要意义。

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Portable, Wireless Potentiostat Sensor for Ultra-Sensitive, Real-Time Detection of 5hmC in Genomic DNA Using Tree-Like Graphene

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Portable, Wireless Potentiostat Sensor for Ultra-Sensitive, Real-Time Detection of 5hmC in Genomic DNA Using Tree-Like Graphene

Aberrant alterations in genomic 5-hydroxymethylcytosine (5hmC), an oxidation product of 5-methylcytosine (5mC) by Ten-eleven translocation (TET) enzymes, are frequently associated with cancers. Quick and precise 5hmC quantification is vital since it is a key biomarker for diagnosis, pathophysiology, and therapy. Here, we present a portable, wireless potentiostat sensor for real-time, ultrasensitive 5hmC-DNA sensing based on a tree-like graphene (teG)-modified screen-printed microelectrode. One-pot electrochemical exfoliation of pencil graphite enabled the cost-effective, eco-friendly, and scalable synthesis of teG, which exhibited high electrical conductivity, excellent electrochemical conductivity, low surface roughness, and high 5hmC-DNA adsorption, surpassing those of pencil graphite (pG) and graphene oxide (GO). The teG-modified gold electrodes exhibited exceptional sensitivity (6.15 × 10^–6 mM^–1 cm^–2), selectivity, and reproducibility, with an ultralow detection limit of 12.6 fM for 5hmC-DNA. The sensor’s performance was validated by quantifying 5hmC levels in genomic DNA from various biological specimens, including primary mouse tissues with altered TET function, mouse hepatocellular carcinoma, and human prostate cancer cell lines. To enhance practicality, a flexible, screen-printed microelectrode on mulberry paper was developed and integrated with a portable, wireless potentiostat powered by the Arduino Nano 33 IoT. Open-circuit potential (OCP)-based detection enabled label-free, real-time monitoring with wireless data transmission to an Android mobile application, successfully differentiating 5hmC levels between cancerous and noncancerous cells. These findings highlight teG’s high surface area, superior charge transport, and scalability, positioning it as a promising platform for next-generation biosensing. The developed sensor provides a rapid, cost-effective, and highly sensitive tool for 5hmC quantification, with significant implications for early cancer diagnostics and treatment.

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来源期刊

ACS Nano 工程技术-材料科学：综合

CiteScore

26.00

自引率

4.10%

发文量

1627

审稿时长

1.7 months

期刊介绍： ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.