Jie Jin, Chao Wang, Ronggui Liu, Rong Zheng, Maohua Deng, Jianfeng Wang, Chunjie Li
{"title":"velezensis LZUR632的潜在遗传特性通过改变根际微生物群落组成和功能基因增强了酒Achnatherum inebrians对Cd胁迫的抗性","authors":"Jie Jin, Chao Wang, Ronggui Liu, Rong Zheng, Maohua Deng, Jianfeng Wang, Chunjie Li","doi":"10.1007/s11104-025-07465-9","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background and aim</h3><p>Heavy metal-resistant plant growth-promoting rhizobacteria can facilitate bioremediation, phytostimulation, and stress reduction in metal-contaminated soil, providing an environmentally friendly method for sustainable agriculture. In this study, we explored the mechanisms underlying the plant growth promotion effects of LZUR632, a strain that enhances the phytoremediation of Cd in drunken horse grass.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>We first isolated a rhizosphere bacterium from the rhizosphere soil of E + <i>A. inebrians</i>, named LZUR632. Then, we co-cultured the LZUR632 and <i>Arabidopsis</i> and <i>A. inebrians</i> seedlings under the different concentrations of CdCl<sub>2</sub>. The whole genome, metabolome, macrogenomic sequences, soil biochemical properties and plant growth response were analyzed.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>Our results indicated inoculation of LZUR632 significantly promoted plant growth and improved Cd stress resistance. And LZUR632 encoded numerous genes required for Cd tolerance and secreted many compounds that contributed to plant growth. Additionally, inoculation of LZUR632 induced taxonomic and functional shifts in the rhizosphere microbial community, increasing plant growth-promoting microbes and nitrogen-fixing microbes that enhanced nutrient assimilation and plant growth. Upregulation of genes encoding lipid metabolism might also aid in stabilizing Cd ions in the rhizosphere soil.</p><h3 data-test=\"abstract-sub-heading\">Conclusions</h3><p>These findings demonstrated the potential of LZUR632-mediated phytoremediation and provided insights into the microbe-induced mechanisms of plant growth promotion in Cd-contaminated soil.</p>","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":"81 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Potential genetic characteristics of Bacillus velezensis LZUR632 enhance the resistance of Achnatherum inebrians to Cd stress by altering rhizosphere microbial community composition and functional genes\",\"authors\":\"Jie Jin, Chao Wang, Ronggui Liu, Rong Zheng, Maohua Deng, Jianfeng Wang, Chunjie Li\",\"doi\":\"10.1007/s11104-025-07465-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Background and aim</h3><p>Heavy metal-resistant plant growth-promoting rhizobacteria can facilitate bioremediation, phytostimulation, and stress reduction in metal-contaminated soil, providing an environmentally friendly method for sustainable agriculture. In this study, we explored the mechanisms underlying the plant growth promotion effects of LZUR632, a strain that enhances the phytoremediation of Cd in drunken horse grass.</p><h3 data-test=\\\"abstract-sub-heading\\\">Methods</h3><p>We first isolated a rhizosphere bacterium from the rhizosphere soil of E + <i>A. inebrians</i>, named LZUR632. Then, we co-cultured the LZUR632 and <i>Arabidopsis</i> and <i>A. inebrians</i> seedlings under the different concentrations of CdCl<sub>2</sub>. The whole genome, metabolome, macrogenomic sequences, soil biochemical properties and plant growth response were analyzed.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>Our results indicated inoculation of LZUR632 significantly promoted plant growth and improved Cd stress resistance. And LZUR632 encoded numerous genes required for Cd tolerance and secreted many compounds that contributed to plant growth. Additionally, inoculation of LZUR632 induced taxonomic and functional shifts in the rhizosphere microbial community, increasing plant growth-promoting microbes and nitrogen-fixing microbes that enhanced nutrient assimilation and plant growth. Upregulation of genes encoding lipid metabolism might also aid in stabilizing Cd ions in the rhizosphere soil.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusions</h3><p>These findings demonstrated the potential of LZUR632-mediated phytoremediation and provided insights into the microbe-induced mechanisms of plant growth promotion in Cd-contaminated soil.</p>\",\"PeriodicalId\":20223,\"journal\":{\"name\":\"Plant and Soil\",\"volume\":\"81 1\",\"pages\":\"\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-04-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant and Soil\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11104-025-07465-9\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-025-07465-9","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Potential genetic characteristics of Bacillus velezensis LZUR632 enhance the resistance of Achnatherum inebrians to Cd stress by altering rhizosphere microbial community composition and functional genes
Background and aim
Heavy metal-resistant plant growth-promoting rhizobacteria can facilitate bioremediation, phytostimulation, and stress reduction in metal-contaminated soil, providing an environmentally friendly method for sustainable agriculture. In this study, we explored the mechanisms underlying the plant growth promotion effects of LZUR632, a strain that enhances the phytoremediation of Cd in drunken horse grass.
Methods
We first isolated a rhizosphere bacterium from the rhizosphere soil of E + A. inebrians, named LZUR632. Then, we co-cultured the LZUR632 and Arabidopsis and A. inebrians seedlings under the different concentrations of CdCl2. The whole genome, metabolome, macrogenomic sequences, soil biochemical properties and plant growth response were analyzed.
Results
Our results indicated inoculation of LZUR632 significantly promoted plant growth and improved Cd stress resistance. And LZUR632 encoded numerous genes required for Cd tolerance and secreted many compounds that contributed to plant growth. Additionally, inoculation of LZUR632 induced taxonomic and functional shifts in the rhizosphere microbial community, increasing plant growth-promoting microbes and nitrogen-fixing microbes that enhanced nutrient assimilation and plant growth. Upregulation of genes encoding lipid metabolism might also aid in stabilizing Cd ions in the rhizosphere soil.
Conclusions
These findings demonstrated the potential of LZUR632-mediated phytoremediation and provided insights into the microbe-induced mechanisms of plant growth promotion in Cd-contaminated soil.
期刊介绍：
Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.
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