Hypoxia and viscosity Co-controlled fluorescence probe for signal amplification imaging of early hypoxia-related diseases

Hypoxia, the phenomenon of decline of oxygen levels in tissues, is a key feature of many diseases and exacerbates the progression of some. At the same time, prolonged hypoxia can cause mitochondrial autophagy and promote viscosity. Therefore, fluorescent probes for detecting hypoxia have been widely reported. However, most of the fluorescence probes used for hypoxia detection have low signal amplification. Therefore, we developed a novel fluorescence probe NF that is co-regulated by hypoxia and viscosity. The probe is capable of rapid response (100 s) with hypoxia marker nitroreductase (NTR) and is capable of signal amplification after viscosity response. Moreover, NF was the first to detect hypoxia in early alcoholic liver injury mice (predate histopathological analysis) and hypoxia in early rheumatoid arthritis.