嗜酸乳杆菌联合HKL通过抑制JAK2-STATI和影响牛磺酸代谢来减轻大鼠结肠炎症

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-04-19 DOI:10.1016/j.jff.2025.106819

Kasimujiang Aximujiang , Kuerbannaimu Kaheman , Jiwei Zhu , Tongshu Shang , Kurexi Yunusi , Xilinguli Wushouer

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引用次数: 0

摘要

方法选取雄性Wistar大鼠，随机分为对照组、UC组、HKL组、Lac组、Lac+HKL组（LH组）和5-ASA组。除对照组外，其他各组均采用 TNBS/乙醇法建立 UC 模型。模型建立成功后，胃内注射相应药物 14 天。通过免疫组化（IHC）检测JAK2、STAT1和STAT3的表达。采用元基因组学技术分析 LH 治疗后 UC 大鼠粪便微生物组的形成和功能变化。结果 IHC结果显示，LH干预后，JAK2和STAT1蛋白表达下调，但STAT3蛋白表达无明显变化；UC组牛磺酸代谢相关基因丰度明显升高，而LH干预后相关基因丰度下降。结论 LH治疗可影响UC大鼠肠道菌群的组成，抑制JAK2-STAT1信号通路，LH治疗可抑制UC大鼠肠道内牛磺酸代谢的异常升高。

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L.acidophilus combined with HKL alleviated colon inflammation in rats by inhibiting JAK2-STATI and affecting taurine metabolism

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L.acidophilus combined with HKL alleviated colon inflammation in rats by inhibiting JAK2-STATI and affecting taurine metabolism

Objective To explore the therapeutic mechanism of L.acidophilus combined with Traditional Chinese Medicine Huan Kui Le(HKL) on ulcerative colitis(UC) by intestinal flora,intestinal immunity and metabolism.

Methods The male Wistar rats were selected and randomly divided into Control group, UC Group, HKL Group, Lac Group, Lac + HKL Group (LH Group), and 5-ASA group. The UC model was established by TNBS/ethanol method in all groups except the Control group. After the model was successfully established, the corresponding drugs were administered intragastrically for 14 days. The expression of JAK2, STAT1, and STAT3 was detected by immunohistochemistry(IHC). The metagenomic technique was used to analyze fecal microbiome formation and function changes in UC rats after LH Tretment. Result The IHC results showed that the expression of JAK2 and STAT1 protein was down-regulated after LH intervention, but the expression of STAT3 protein was not significantly changed.The abundance of genes related to taurine metabolism was significantly higher in the UC group, while the abundance of related genes decreased after LH intervention. GT45, GH114, GH104, and PL3 were highly expressed in the UC group, while the expression was downregulated after LH intervention.Staphylococcus is a major contributor to the expression of carbohydrate related genes.Conclusion The LH Treatment can affect the composition of intestinal flora in UC rats and inhibit JAK2-STAT1 signal pathway.LH treatment can inhibit the abnormal increase of taurine metabolism in the gut of UC rats.

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.