具有多色性能的j聚集荧光纳米粒子使LFIA平台敏感

IF 9.6 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-04-18 DOI:10.1021/acs.nanolett.5c01012

Gan Zhang, Keyang Lai, Lilin Zhang, Xiaocui Lai, Shengliang Deng, Juan Peng, Weihua Lai

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引用次数: 0

摘要

传统的侧流免疫分析法（LFIA）由于信号读数单一且灵敏度较差，其应用仍然具有挑战性。在这项工作中，我们开发了具有多色性能的j聚集荧光纳米颗粒（JNPs），并将其作为报告者，在LFIA平台上同时灵敏地检测T-2和AFB1毒素。在纳米粒子的强约束作用下，JNPs的发射区从~ 545 nm红移到~ 620 nm，表现出强烈的j聚集现象。随后，采用两种具有绿色和红色发射特性的JNPs构建了多重LFIA平台（JNPs -LFIA），用于T-2和AFB1毒素的现场定量检测，其检出限分别为0.645 ng mL-1和0.0035 ng mL-1，分别比AuNPs-LFIA低3.5倍和12.2倍。总之，本研究为多色免疫探针的设计提供了一种有效的策略，并促进了报告蛋白在生物传感应用中的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

J-Aggregated Fluorescence Nanoparticles with Multichromatic Performance Enable Sensitive LFIA Platform

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J-Aggregated Fluorescence Nanoparticles with Multichromatic Performance Enable Sensitive LFIA Platform

The application of conventional lateral flow immunoassay (LFIA) remains challenging due to its single signal readout and poor sensitivity. In this work, we developed J-aggregated fluorescence nanoparticles (JNPs) with multichromatic performance and employed them as reporters for the simultaneous and sensitive detection of T-2 and AFB1 toxins on the LFIA platform. Upon the strong confinement effect of nanoparticles, the emission region of JNPs was red-shifted from ∼545 to ∼620 nm, exhibiting a strong J-aggregation phenomenon. Subsequently, two JNPs with green emission and red emission were employed for the development of multiplexed LFIA platform (JNPs–LFIA) for on-site quantitative detection of T-2 and AFB1 toxins, with the limits of detection of 0.645 ng mL^–1 and 0.0035 ng mL^–1, which were 3.5-fold and 12.2-fold lower than those of AuNPs-LFIA, respectively. Overall, this work provides an efficient strategy for the design of multichromatic immunoprobes and promotes the development of reporters in biosensing application.

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.