空间表观基因组、转录组和细胞系的统一分子方法

IF 9.1 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Yung-Hsin Huang, Julia A. Belk, Ruochi Zhang, Natasha E. Weiser, Zachary Chiang, Matthew G. Jones, Paul S. Mischel, Jason D. Buenrostro, Howard Y. Chang
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引用次数: 0

摘要

空间表观基因组学和多组学可以提供对细胞状态的细粒度洞察,但它们的广泛采用受到定制幻灯片和捕获每种数据模式化学成分的要求的限制。在这里,我们提出了利用测序(SPACE-seq)对可接近的染色质、细胞系和基因表达进行空间分析的方法,这种方法利用多腺嘌呤尾表观基因组文库,使用标准的全转录组试剂实现方便的空间多组学。将SPACE-seq应用于人类胶质母细胞瘤标本,我们揭示了肿瘤微环境的状态,染色体外DNA拷贝数,并鉴定了假定的线粒体DNA变异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unified molecular approach for spatial epigenome, transcriptome, and cell lineages
Spatial epigenomics and multiomics can provide fine-grained insights into cellular states but their widespread adoption is limited by the requirement for bespoke slides and capture chemistries for each data modality. Here, we present SPatial assay for Accessible chromatin, Cell lineages, and gene Expression with sequencing (SPACE-seq), a method that utilizes polyadenine-tailed epigenomic libraries to enable facile spatial multiomics using standard whole transcriptome reagents. Applying SPACE-seq to a human glioblastoma specimen, we reveal the state of the tumor microenvironment, extrachromosomal DNA copy numbers, and identify putative mitochondrial DNA variants.
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来源期刊
CiteScore
19.00
自引率
0.90%
发文量
3575
审稿时长
2.5 months
期刊介绍: The Proceedings of the National Academy of Sciences (PNAS), a peer-reviewed journal of the National Academy of Sciences (NAS), serves as an authoritative source for high-impact, original research across the biological, physical, and social sciences. With a global scope, the journal welcomes submissions from researchers worldwide, making it an inclusive platform for advancing scientific knowledge.
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