辅助生殖技术后卵泡液中维生素D代谢物24,25(OH)2D3的浓度与活产率呈正相关

IF 2.7 2区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Steroid Biochemistry and Molecular Biology Pub Date : 2025-04-15 DOI:10.1016/j.jsbmb.2025.106764

Ireen Kooij , Rune Holt , Li Juel Mortensen , Mette Lorenzen , Ursula Bentin-Ley , Hans Krog , Anders Hayden Berg , Anders Juul , Stine Gry Kristensen , Anne Jørgensen , Martin Blomberg Jensen

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引用次数: 0

摘要

目的探讨卵泡液中维生素D代谢物浓度与辅助生殖技术（ART）治疗效果的关系。前瞻性队列研究包括116名接受体外受精（IVF）或细胞质内精子注射（ICSI）治疗的妇女。结果卵泡液中维生素D代谢产物25OHD3、1,25(OH)2D3和24,25(OH)2D3均可检出。卵泡液中24,25(OH)2D3代谢物的浓度高于相应的血清值，而1,25(OH)2D3代谢物的浓度则相反。局部转化是合理的，因为维生素D激活酶（CYP2R1和CYP27B1）以及维生素D受体（VDR）在发育中的卵泡中高度表达。妇女取得了活产29 %更高的24日,25 (OH) 2 d3和浓度高出15 % 25 ohd3卵泡液(18 ± 7.9和14 ± 6.2 nmol / L p = 0.008和71 ± 22和62 ± 18 nmol / L, p = 0.025,分别)相比,女性没有实现活产。此外，卵泡液1,25(OH)2D3/24,25(OH)2D3比例低（≤15 %）的妇女的活产率高于中（16-84 %）或高（≥85 %）比例的妇女（活产率分别为53 %对29 %和12 %，p = 0.032）。结论卵泡液中高水平的24,25(OH)2D3和低水平的1,25(OH)2D3/24,25(OH)2D3比值与接受IVF/ICSI治疗的妇女的活产率升高有关。全身或卵巢卵泡内维生素D代谢物的转化可能影响ART的结果。需要进一步的研究来支持这一初步研究的发现，并确定卵巢维生素D代谢产物的调节因子。

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Follicular fluid concentrations of the vitamin D metabolite 24,25(OH)2D3 are positively associated with live birth rate after assisted reproductive technology

Objective

To investigate the relationship between concentrations of vitamin D metabolites in follicular fluid and outcome of assisted reproductive technology (ART) treatment.

Design

Prospective cohort study including 116 women undergoing in vitro fertilization (IVF) or intra cytoplasmic sperm injection (ICSI) treatment.

Results

All measured vitamin D metabolites 25OHD₃, 1,25(OH)₂D₃ and 24,25(OH)₂D₃ are detectable in follicular fluid. Follicular fluid concentration of the 24,25(OH)₂D₃ metabolite was higher than corresponding serum values, while the opposite phenomenon was observed for the 1,25(OH)₂D₃ metabolite. Local conversion is plausible as the vitamin D activating enzymes (CYP2R1 and CYP27B1) as well as the vitamin D receptor (VDR) are highly expressed in the developing follicle. Women who achieved a live birth had 29 % higher 24,25(OH)₂D₃ and 15 % higher 25OHD₃ concentrations in their follicular fluid (18 ± 7.9 versus 14 ± 6.2 nmol/L, p = 0.008 and 71 ± 22 versus 62 ± 18 nmol/L, p = 0.025, respectively) compared to women not achieving a live birth. Moreover, women with a low (≤ 15 %) follicular fluid 1,25(OH)₂D₃/24,25(OH)₂D₃ ratio had a higher live birth rate compared to women with a medium (16–84 %) or high (≥ 85 %) ratio (live birth rate: 53 % vs 29 % and 12 %, respectively, p = 0.032).

Conclusion

This study reveals that high levels of 24,25(OH)₂D₃ and low levels of the 1,25(OH)₂D₃/24,25(OH)₂D₃ ratio in follicular fluid are associated with increased live birth rates in women undergoing IVF/ICSI treatment. Conversion of vitamin D metabolites systemically or in the ovarian follicle may affect ART outcome. Further studies are warranted to support the findings from this pilot study and identify regulators of ovarian vitamin D metabolites.

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来源期刊

Journal of Steroid Biochemistry and Molecular Biology 生物-内分泌学与代谢

CiteScore

8.60

自引率

2.40%

发文量

113

审稿时长

46 days

期刊介绍： The Journal of Steroid Biochemistry and Molecular Biology is devoted to new experimental and theoretical developments in areas related to steroids including vitamin D, lipids and their metabolomics. The Journal publishes a variety of contributions, including original articles, general and focused reviews, and rapid communications (brief articles of particular interest and clear novelty). Selected cutting-edge topics will be addressed in Special Issues managed by Guest Editors. Special Issues will contain both commissioned reviews and original research papers to provide comprehensive coverage of specific topics, and all submissions will undergo rigorous peer-review prior to publication.