奇异变形杆菌的基因组分析:揭示全球流行病学和抗菌素耐药性传播-公共卫生和生物安全面临的新挑战

IF 10.3 1区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Shaqiu Zhang , Qianlong Li , Mingshu Wang , Renyong Jia , Shun Chen , Mafeng Liu , Dekang Zhu , Xinxin Zhao , Ying Wu , Qiao Yang , Juan Huang , Xumin Ou , Di Sun , Bin Tian , Yu He , Zhen Wu , Anchun Cheng
{"title":"奇异变形杆菌的基因组分析:揭示全球流行病学和抗菌素耐药性传播-公共卫生和生物安全面临的新挑战","authors":"Shaqiu Zhang ,&nbsp;Qianlong Li ,&nbsp;Mingshu Wang ,&nbsp;Renyong Jia ,&nbsp;Shun Chen ,&nbsp;Mafeng Liu ,&nbsp;Dekang Zhu ,&nbsp;Xinxin Zhao ,&nbsp;Ying Wu ,&nbsp;Qiao Yang ,&nbsp;Juan Huang ,&nbsp;Xumin Ou ,&nbsp;Di Sun ,&nbsp;Bin Tian ,&nbsp;Yu He ,&nbsp;Zhen Wu ,&nbsp;Anchun Cheng","doi":"10.1016/j.envint.2025.109487","DOIUrl":null,"url":null,"abstract":"<div><div>Given the escalating public health threat posed by<!--> <em>Proteus mirabilis</em> <!-->(<em>P. mirabilis</em>) and its rapidly evolving drug resistance, it is imperative to elucidate its global epidemiology and resistance mechanisms through a comprehensive genomic lens. As of August 2024, 3,403 high-quality<!--> <em>P. mirabilis</em> <!-->genomes were retrieved from public databases (total 3,752), spanning 58 countries/regions, with the United States showing the highest report rate (52.51 %). Human-derived isolates, particularly from urine (34.47 %), were the primary source. A total of 239 antibiotic resistance genes (ARGs) were identified in <em>P. mirabilis</em>, with β-lactamase and carbapenemase genes being particularly widespread and isolates from China harboring the highest ARG counts. Globally,<!--> <em>P. mirabilis</em> <!-->isolates were categorized into 17 distinct clusters, with U.S. isolates showing the widest phylogenetic spread. Minimal SNP variations among isolates from different countries and hosts suggest transnational and cross-host clonal propagation. Frequent clonal transmission was also observed among diverse hosts and clinical sources.<!--> <em>P. mirabilis</em> <!-->carries numerous integrative and conjugative elements (ICEs), some facilitating ARG dissemination (n = 215). Prophages, though ubiquitous, contributed minimally to ARG spread. Spearman’s analysis revealed significant correlations between ARGs and insertion sequences (ISs), replicons, and ICEs. Ancestral state analysis indicated prophages were mainly acquired horizontally, while other mobile genetic elements (MGEs) were largely clonally transmitted. This study provides the first comprehensive genomic analysis of<!--> <em>P. mirabilis</em>’s global resistance landscape, highlighting the need to designate it as a novel antimicrobial resistance indicator and implement long-term surveillance.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109487"},"PeriodicalIF":10.3000,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genomic analysis of Proteus mirabilis: Unraveling global epidemiology and antimicrobial resistance dissemination − emerging challenges for public health and biosecurity\",\"authors\":\"Shaqiu Zhang ,&nbsp;Qianlong Li ,&nbsp;Mingshu Wang ,&nbsp;Renyong Jia ,&nbsp;Shun Chen ,&nbsp;Mafeng Liu ,&nbsp;Dekang Zhu ,&nbsp;Xinxin Zhao ,&nbsp;Ying Wu ,&nbsp;Qiao Yang ,&nbsp;Juan Huang ,&nbsp;Xumin Ou ,&nbsp;Di Sun ,&nbsp;Bin Tian ,&nbsp;Yu He ,&nbsp;Zhen Wu ,&nbsp;Anchun Cheng\",\"doi\":\"10.1016/j.envint.2025.109487\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Given the escalating public health threat posed by<!--> <em>Proteus mirabilis</em> <!-->(<em>P. mirabilis</em>) and its rapidly evolving drug resistance, it is imperative to elucidate its global epidemiology and resistance mechanisms through a comprehensive genomic lens. As of August 2024, 3,403 high-quality<!--> <em>P. mirabilis</em> <!-->genomes were retrieved from public databases (total 3,752), spanning 58 countries/regions, with the United States showing the highest report rate (52.51 %). Human-derived isolates, particularly from urine (34.47 %), were the primary source. A total of 239 antibiotic resistance genes (ARGs) were identified in <em>P. mirabilis</em>, with β-lactamase and carbapenemase genes being particularly widespread and isolates from China harboring the highest ARG counts. Globally,<!--> <em>P. mirabilis</em> <!-->isolates were categorized into 17 distinct clusters, with U.S. isolates showing the widest phylogenetic spread. Minimal SNP variations among isolates from different countries and hosts suggest transnational and cross-host clonal propagation. Frequent clonal transmission was also observed among diverse hosts and clinical sources.<!--> <em>P. mirabilis</em> <!-->carries numerous integrative and conjugative elements (ICEs), some facilitating ARG dissemination (n = 215). Prophages, though ubiquitous, contributed minimally to ARG spread. Spearman’s analysis revealed significant correlations between ARGs and insertion sequences (ISs), replicons, and ICEs. Ancestral state analysis indicated prophages were mainly acquired horizontally, while other mobile genetic elements (MGEs) were largely clonally transmitted. This study provides the first comprehensive genomic analysis of<!--> <em>P. mirabilis</em>’s global resistance landscape, highlighting the need to designate it as a novel antimicrobial resistance indicator and implement long-term surveillance.</div></div>\",\"PeriodicalId\":308,\"journal\":{\"name\":\"Environment International\",\"volume\":\"199 \",\"pages\":\"Article 109487\"},\"PeriodicalIF\":10.3000,\"publicationDate\":\"2025-04-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environment International\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0160412025002387\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environment International","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0160412025002387","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

鉴于奇异变形杆菌(P. mirabilis)对公共卫生的威胁不断升级及其耐药性的迅速演变,有必要通过全面的基因组视角阐明其全球流行病学和耐药机制。截至2024年8月,共从公共数据库中检索到3403个高质量的奇异假单胞菌基因组(共3752个),分布在58个国家/地区,其中美国的报告率最高(52.51 %)。人类来源的分离株,特别是尿液(34.47 %)是主要来源。共鉴定出239个抗生素耐药基因,其中β-内酰胺酶和碳青霉烯酶基因分布最为广泛,中国分离物中ARG数量最多。在全球范围内,P. mirabilis分离株被分为17个不同的簇,其中美国分离株显示出最广泛的系统发育分布。来自不同国家和宿主的分离物之间的最小SNP差异表明跨国和跨宿主克隆繁殖。在不同宿主和临床来源之间也观察到频繁的克隆传播。P. mirabilis携带许多整合和共轭因子(ICEs),其中一些促进ARG传播(n = 215)。噬菌体虽然无处不在,但对ARG传播的贡献很小。Spearman的分析显示ARGs与插入序列(ISs)、复制子和ICEs之间存在显著相关性。祖先状态分析表明,前噬菌体主要是水平获得的,而其他移动遗传元件(MGEs)主要是无性传播的。这项研究首次提供了mirabilis全球耐药格局的全面基因组分析,强调了将其指定为新型抗菌素耐药指标和实施长期监测的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genomic analysis of Proteus mirabilis: Unraveling global epidemiology and antimicrobial resistance dissemination − emerging challenges for public health and biosecurity

Genomic analysis of Proteus mirabilis: Unraveling global epidemiology and antimicrobial resistance dissemination − emerging challenges for public health and biosecurity

Genomic analysis of Proteus mirabilis: Unraveling global epidemiology and antimicrobial resistance dissemination − emerging challenges for public health and biosecurity
Given the escalating public health threat posed by Proteus mirabilis (P. mirabilis) and its rapidly evolving drug resistance, it is imperative to elucidate its global epidemiology and resistance mechanisms through a comprehensive genomic lens. As of August 2024, 3,403 high-quality P. mirabilis genomes were retrieved from public databases (total 3,752), spanning 58 countries/regions, with the United States showing the highest report rate (52.51 %). Human-derived isolates, particularly from urine (34.47 %), were the primary source. A total of 239 antibiotic resistance genes (ARGs) were identified in P. mirabilis, with β-lactamase and carbapenemase genes being particularly widespread and isolates from China harboring the highest ARG counts. Globally, P. mirabilis isolates were categorized into 17 distinct clusters, with U.S. isolates showing the widest phylogenetic spread. Minimal SNP variations among isolates from different countries and hosts suggest transnational and cross-host clonal propagation. Frequent clonal transmission was also observed among diverse hosts and clinical sources. P. mirabilis carries numerous integrative and conjugative elements (ICEs), some facilitating ARG dissemination (n = 215). Prophages, though ubiquitous, contributed minimally to ARG spread. Spearman’s analysis revealed significant correlations between ARGs and insertion sequences (ISs), replicons, and ICEs. Ancestral state analysis indicated prophages were mainly acquired horizontally, while other mobile genetic elements (MGEs) were largely clonally transmitted. This study provides the first comprehensive genomic analysis of P. mirabilis’s global resistance landscape, highlighting the need to designate it as a novel antimicrobial resistance indicator and implement long-term surveillance.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Environment International
Environment International 环境科学-环境科学
CiteScore
21.90
自引率
3.40%
发文量
734
审稿时长
2.8 months
期刊介绍: Environmental Health publishes manuscripts focusing on critical aspects of environmental and occupational medicine, including studies in toxicology and epidemiology, to illuminate the human health implications of exposure to environmental hazards. The journal adopts an open-access model and practices open peer review. It caters to scientists and practitioners across all environmental science domains, directly or indirectly impacting human health and well-being. With a commitment to enhancing the prevention of environmentally-related health risks, Environmental Health serves as a public health journal for the community and scientists engaged in matters of public health significance concerning the environment.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信