3-吲哚乙酸通过线粒体ros介导的IL-17/CXCL10/TNF-α信号通路对双酚s诱导的肠道屏障功能障碍的保护作用

IF 10.3 1区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Jing Wang , Guolei Niu , Huanzhuo Mai , Xianan Zhang , Jiahao Zhu , Bin Li , Yushuang Gao , Tengkai Huang , Qingtao Meng , Rui Chen
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引用次数: 0

摘要

双酚S (BPS)已广泛应用于消费品的制造。BPS主要通过食物和水进入人体,经口接触以胃肠道为目标。然而,它的安全性仍然存在争议,需要进一步调查。在这项研究中,我们旨在评估在没有明显病理损伤的情况下BPS是否对身体产生有害影响。我们的研究结果显示,虽然BPS不会导致明显的组织病理学损伤,但它会引起肠屏障功能障碍。此外,利用NCM460细胞和人类来源的结肠直肠类器官进行的体外研究表明,BPS暴露可诱导肠内分泌细胞(EECs)线粒体活性氧(ROS)水平,上调炎症介质TNF-α和CXCL10的表达。使用dss诱导的结肠炎小鼠模型,发现BPS暴露加剧了肠道炎症性疾病的进展。单细胞数据库分析显示,炎症性肠病(IBD)患者中肠内分泌细胞(EECs)功能蛋白CHGA的表达显著降低。CHGA的表达与IL17的表达呈显著负相关。值得注意的是,补充3-吲哚乙酸可有效减轻BPS引起的肠道损伤。这些发现强调了线粒体氧化应激和IL-17/CXCL10/TNF-α信号在bps诱导的肠道损伤中的作用,并证明了3-吲哚乙酸在减轻这些影响方面的治疗潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The protective role of 3-Indoleglyoxylic acid in Bisphenol S-induced intestinal barrier dysfunction via mitochondrial ROS-Mediated IL-17/CXCL10/TNF-α signaling
Bisphenol S (BPS) has become extensively used in the manufacturing of consumer products. BPS mainly enters the body through food and water, with oral exposure targeting the gastrointestinal tract. However, its safety profile remains contentious and warrants further investigation. In this study, we aimed to assess whether BPS exerts harmful effects on the body in the absence of overt pathological damage. Our results revealed that although BPS did not lead to significant histopathological damage, it induced intestinal barrier dysfunction. Additionally, in vitro investigations utilizing NCM460 cells and human-derived colorectal organoids demonstrated that BPS exposure induced mitochondrial reactive oxygen species (ROS) levels in intestinal endocrine cells (EECs), upregulating the expression of inflammatory mediators TNF-α and CXCL10. Using a DSS-induced colitis mouse model, it was found that BPS exposure exacerbates the progression of intestinal inflammatory diseases. Analysis of single-cell databases demonstrated a significant reduction in the expression of CHGA, a functional protein of enteroendocrine cells (EECs), in patients with inflammatory bowel disease (IBD). The expression of CHGA showed a significant negative correlation with the expression of IL17. Notably, supplementation with 3-Indoleglyoxylic acid effectively mitigates the intestinal damage induced by BPS. These findings highlight the role of mitochondrial oxidative stress and IL-17/CXCL10/TNF-α signaling in BPS-induced intestinal damage and demonstrate the therapeutic potential of 3-Indoleglyoxylic acid in mitigating these effects.
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来源期刊
Environment International
Environment International 环境科学-环境科学
CiteScore
21.90
自引率
3.40%
发文量
734
审稿时长
2.8 months
期刊介绍: Environmental Health publishes manuscripts focusing on critical aspects of environmental and occupational medicine, including studies in toxicology and epidemiology, to illuminate the human health implications of exposure to environmental hazards. The journal adopts an open-access model and practices open peer review. It caters to scientists and practitioners across all environmental science domains, directly or indirectly impacting human health and well-being. With a commitment to enhancing the prevention of environmentally-related health risks, Environmental Health serves as a public health journal for the community and scientists engaged in matters of public health significance concerning the environment.
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