为调控自噬而磷酸化的 FOX 转录因子有助于硬皮病菌子实体的发育

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-04-18 DOI:10.1111/nph.70151

Genglin Zhu, Qi Zuo, Sirui Liu, Peiyi Zheng, Yanhua Zhang, Xianghui Zhang, Jeffrey A. Rollins, Jinliang Liu, Hongyu Pan

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引用次数: 0

摘要

自噬是真核细胞降解自身成分的循环过程，而子实体（性结构）是某些植物病原真菌开始侵染循环的必要结构。然而，植物病原真菌自噬和自噬调节有性生殖的转录调控尚不清楚。本文报道了植物病原真菌的自噬转录与子实体发育之间的联系。Sclerotinia sclerotiorum （Ss）中的叉头盒转录因子（foxtf） SsFoxE2与ATG基因的启动子结合，从而促进ATG基因的转录。SsFoxE2被amp激活的蛋白激酶（AMPK） SsSnf1磷酸化，磷酸化的SsFoxE2与（翻译控制的肿瘤蛋白）SsTctp1相互作用，导致SsFoxE2的稳定性和ATG转录活性增强。重要的是，SsFoxE2对自噬的调控影响着泛素化系统的平衡和子实体的早期发育，直接决定着植物病害的发生和流行。此外，FOX TF与ATG基因启动子的转录结合在植物致病真菌中是保守的。综上所述，我们的研究结果为植物病原真菌的病原体起始提供了新的见解，并将其与植物病原体中其他自噬调节的过程联系起来。

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A FOX transcription factor phosphorylated for regulation of autophagy facilitates fruiting body development in Sclerotinia sclerotiorum

Autophagy is a recycling process by which eukaryotic cells degrade their own components, and the fruiting body (sexual structure) is a necessary structure for some plant pathogenic fungi to start the infection cycle. However, the transcriptional regulation of plant pathogenic fungal autophagy and autophagy regulating sexual reproduction remains elusive.
Here, we provide the report linking autophagy transcription and fruiting body development in phytopathogenic fungi. The forkhead box transcription factor (FOX TF) SsFoxE2 in Sclerotinia sclerotiorum (Ss) binds to the promoters of ATG genes, thus promoting their transcription. SsFoxE2 is phosphorylated by AMP-activated protein kinase (AMPK) SsSnf1, and the phosphorylated SsFoxE2 interacts with (translationally controlled tumor protein) SsTctp1, leading to enhanced stability and ATG transcription activity of SsFoxE2.
Importantly, the regulation of autophagy by SsFoxE2 affects the balance of the ubiquitination system and the early development of the fruiting body, which directly determines the occurrence and prevalence of plant disease. Furthermore, transcriptional binding of FOX TF to ATG gene promoters is conserved in phytopathogenic fungi.
Taken together, our results bring new insights into pathogen initiation in phytopathogenic fungi and connect it to other autophagy-regulated processes in plant pathogens.

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.