诱导手性过氧化物酶纳米酶活性的多酚双金属对映体结构，用于胃癌对映体的鉴定

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-04-09 DOI:10.1016/j.bios.2025.117449

Qiu-hong Yao , Mei-huang Zeng , Chen Zhang , Feng Zheng , Jing-wen Jin , Ting-xiu Ye , Yi-ting Wang , Xiao-mei Chen , Zhi-yong Guo , Xi Chen

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引用次数: 0

摘要

胃癌（GC）患者生物体液中d -丙氨酸（D-Ala）和d -脯氨酸（D-Pro）水平显著升高，被认为是胃癌的潜在生物标志物。因此，监测GC患者体液中D-Pro和D-Ala的浓度具有重要意义。本研究构建了一种基于双金属异质结构纳米酶（3-MPBA: Co-TA@Ag NPs）的高灵敏度表面增强拉曼散射（SERS）传感器，用于气相色谱生物体液中D-AAs的检测。由于Ag-Co双金属双活性中心的形成，电子转移行为加速了中性pH条件下硼羟基的电离，同时D-Ala与D-Pro和3-MPBA之间发生了超强劲的羟基醇酯化反应。通过简单检测不同浓度和样品的不同吸光度强度，构建了无创检测D-Pro/Ala的传感器，具有良好的特异性和效率。D-Pro/Ala的检测限（LOD）分别为2.37 μM和2.15 μM。此外，基于气相色谱生物液中D-Pro/Ala的差异水平，该方法还可以实现唾液样品与D-Pro/Ala的鉴别，在气相色谱诊断中具有很大的潜力。

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Polyphenol briged bimetallic-heterostructure inducing chiral peroxidase nanozyme activity for enantiomers identification in gastric cancer

_D-alanine (_D-Ala) and _D-proline(_D-Pro) levels dramatically increase in the bio-fluids of gastric cancer (GC) patients, which are suggested to be potential biomarkers associated with GC. Therefore, monitoring the concentrations of _D-Pro and _D-Ala in the body fluids of GC patients is of great significance. In this study, a highly sensitive Surface Enhanced Raman Scattering (SERS) sensor has constructed based on bimetallic heterostructure nanoenzyme (3-MPBA: Co-TA@Ag NPs) for _D-AAs detection in GC bio-fluids. Attributing to the formation of Ag-Co bimetallic dual active centers, the electron transfer behaviors accelerated boron hydroxyl group ionization in neutral pH, as well as the ultra-robust hydroxyl alcohol esterification reaction occurred between _D-Ala and _D-Pro and 3-MPBA. By simply testing different absorbance intensities at various concentrations and sample, a sensor for noninvasive detection of _D-Pro/Ala is constructed with good specificity and efficiency. Besides, the quantitative determination of _D-Pro/Ala (limits of detection (LOD) 2.37 μM and 2.15 μM for _D-Pro/Ala. Furthermore, based on the differential level of _D-Pro/Ala in GC bio-fluids, the differentiation of saliva samples with _D-Pro/Ala can also be implemented by the proposed method, showing great potential in GC diagnosis.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.