狗气源性纳米囊泡（GqDNVs）促进MC3T3-E1细胞增殖，促进骨折愈合

IF 6.7 1区医学 Q1 CHEMISTRY, MEDICINAL

Phytomedicine Pub Date : 2025-04-11 DOI:10.1016/j.phymed.2025.156755

Shiyin Xu , Zixuan Zhang , Xiaolei Zhou , Yuxiao Liao , Zhao Peng , Zitong Meng , Andreas K. Nüssler , Liang Ma , Hui Xia , Liegang Liu , Wei Yang

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引用次数: 0

摘要

枸杞（lycium barbarum L.），又称勾气，是一种传统的中草药，被广泛应用于保健品和临床治疗中。其强健筋骨的功效早已被记载在医学经典中。此外，植物外泌体样纳米囊泡（PELNVs）因其生物学特性而受到越来越多的关注。因此，我们拟探讨狗气衍生纳米囊泡（GqDNVs）在骨折愈合中的功能、调控作用及其机制。方法本研究采用蔗糖密度梯度差示超离心分离gqdnv。采用CCK-8法、ALP活性测定法和细胞划痕法评价GqDNVs对MC3T3-E1细胞增殖和分化的影响。此外，利用骨折小鼠模型，我们利用Micro-CT、免疫染色和组织学分析来全面评估gqdnv对小鼠骨折愈合的影响。结果gqdnv刺激细胞活力，提高ALP活性，促进细胞成骨蛋白（OPN、ALP、RUNX2）表达。随后，在小鼠骨折模型中，gqdnv治疗组注射28 d后，骨小梁厚度和骨髓密度均增加。同时，14和28 d后，OPN和BGP的表达均显著升高。治疗14天后，p-PI3K/PI3K、p-Akt/Akt、p-mTOR/mTOR、p-4EBP1/4EBP1、p-p70S6K/ p70S6K表达均升高。结论sgqdnv能有效促进MC3T3-E1细胞的增殖和分化。此外，gqdnv可以促进骨折愈合，这与PI3K/Akt/mTOR/p70S6K/4EBP1信号通路有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Gouqi-derived Nanovesicles (GqDNVs) Promoted MC3T3-E1 Cells Proliferation and Improve Fracture Healing

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Gouqi-derived Nanovesicles (GqDNVs) Promoted MC3T3-E1 Cells Proliferation and Improve Fracture Healing

Background

Lycium barbarum L., also known as Gouqi, a traditional Chinese herbal medicine, is widely utilized in health care products and clinical therapies. Its muscle and bone strengthening efficacy has been recorded in medical classics for a long time. In addition, plant exosome-like nanovesicles (PELNVs) have attracted more and more attention owing to their biological traits. Therefore, we intended to explore the functions, regulatory role, and underlying mechanism of Gouqi-derived Nanovesicles (GqDNVs) on fracture healing.

Methods

In this study, we employed the sucrose density gradient differential ultracentrifugation to isolate GqDNVs. The effects of GqDNVs on the proliferation and differentiation of MC3T3-E1 cells were evaluated using the CCK-8 assay, ALP activity measurement, and cell scratch assay. Additionally, leveraging a fracture mouse model, we utilized Micro-CT, immunological staining, and histologic analyses to comprehensively assess the impact of GqDNVs on fracture healing in mice.

Results

GqDNVs stimulated cell viability, increased ALP activity, and promoted cellular osteogenic protein expression (OPN, ALP, and RUNX2). Subsequently, in the mouse fracture model, trabecular thickness, and bone marrow density were increased in the GqDNVs treatment group after 28 days of injection. Meanwhile, the expressions of OPN and BGP were significantly elevated after both 14 and 28 days. Additionally, the expressions of p-PI3K/PI3K, p-Akt/Akt, p-mTOR/mTOR, p-4EBP1/4EBP1 and p-p70S6K/ p70S6K were also increased after14 days of treatment.

Conclusions

GqDNVs effectively promoted the proliferation and differentiation of MC3T3-E1 cells. Furthermore, GqDNVs could improve fracture healing, which is associated with PI3K/Akt/mTOR/p70S6K/4EBP1 signaling pathway.

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来源期刊

Phytomedicine 医学-药学

CiteScore

10.30

自引率

5.10%

发文量

670

审稿时长

91 days

期刊介绍： Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.