纳米无定形磷酸钙修饰的生物活性修复体与牙本质再矿化的比较研究

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-04-18 DOI:10.1016/j.jobcr.2025.04.009

Garima Tyagi , Sakshi Jain , Shivani Deshwal , Shubham Singh , Nikita Poonia , Shivangi Sharma

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引用次数: 0

摘要

目的现代龋齿管理强调微创技术，通过平衡pH值和矿物质离子可用性来促进再矿化。生物活性修复材料，包括giomer （Beautiful II, SHOFU Dental GmbH，日本）和ACTIVA Bioactive (Pulpdent Corp, Watertown， MA， USA)，释放氟化物、钙和磷酸盐来增强牙本质再矿化。纳米无定形磷酸钙（NACP）是一种高活性矿物，由于其高表面积和溶解度，具有已证实的再矿化能力。NACP与生物活性物质的协同作用还有待进一步研究。本研究利用能量色散x射线光谱（EDX）微量分析和Knoop硬度值（KHN）评估了与NACP结合和不结合的异构体和ACTIVA BioACTIVE的再矿化潜力。材料与方法采用4种复合材料：Beautifil II、Beautifil II + NACP、ACTIVA BioACTIVE、ACTIVA BioACTIVE + NACP修复非龋牙釉质脱矿牙本质龋洞。用EDX分析矿物含量（Ca， P， F），用KHN测定显微硬度。统计分析包括方差分析（ANOVA）和事后Tukey检验。结果NACP显著增加了钙和磷酸盐的沉积，其中ACTIVA BioACTIVE与NACP的再矿化作用最高（Ca/P比为2.16）。在聚合物基材料中，氟化物强度较高，促进了氟磷灰石的形成。KHN分析显示，添加NACP的Beautifil II硬度最高，而添加NACP的ACTIVA BioACTIVE硬度降低，但矿物质含量增加。结论NACP在异构体和ACTIVA BioACTIVE中的掺入促进了钙和磷酸盐离子的积累，导致矿化增强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Comparative study of dentin remineralization with Nano-amorphous calcium phosphate-modified bioactive restoratives

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Comparative study of dentin remineralization with Nano-amorphous calcium phosphate-modified bioactive restoratives

Objective

Modern caries management emphasizes minimally invasive techniques to promote remineralization through a balanced pH and mineral ion availability. Bioactive restorative materials, including giomer (Beautiful II, SHOFU Dental GmbH, Japan), and ACTIVA BioACTIVE (Pulpdent Corp., Watertown, MA, USA), release fluoride, calcium, and phosphate to enhance dentin remineralization. Nano-amorphous calcium phosphate (NACP) is a highly reactive mineral with proven remineralization capabilities due to its high surface area and solubility. The synergistic effects of NACP and the bioactive materials remain underexplored. This study evaluates the remineralization potential of giomer and ACTIVA BioACTIVE with and without NACP integration using energy-dispersive X-ray spectroscopy (EDX) microanalysis and Knoop hardness number (KHN) assessments.

Materials and methods

Four restorative composite materials: Beautifil II, Beautifil II with NACP, ACTIVA BioACTIVE, and ACTIVA BioACTIVE with NACP, were evaluated on demineralized dentin cavities created in non-carious molars. The mineral content (Ca, P, F) was analyzed via EDX, and the microhardness was measured using KHN. Statistical analyses included analysis of variance (ANOVA) and post hoc Tukey tests.

Results

NACP significantly increased calcium and phosphate deposition, with ACTIVA BioACTIVE with NACP demonstrating the highest remineralization (Ca/P ratio: 2.16). Fluoride intensities were higher in the giomer-based materials, promoting fluorapatite formation. KHN analysis revealed that Beautifil II with NACP exhibited the highest hardness, whereas ACTIVA BioACTIVE with NACP showed reduced hardness despite enhanced mineral content.

Conclusion

The incorporation of NACP into giomer and ACTIVA BioACTIVE facilitated the accumulation of calcium and phosphate ions, leading to enhanced mineralization.

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.