颗粒细胞转录同样受到超排卵和衰老的影响,并预测早期胚胎轨迹

IF 15.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Klaudija Daugelaite, Perrine Lacour, Ivana Winkler, Marie-Luise Koch, Anja Schneider, Nina Schneider, Francesca Coraggio, Alexander Tolkachov, Xuan Phuoc Nguyen, Adriana Vilkaite, Julia Rehnitz, Duncan T. Odom, Angela Goncalves
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引用次数: 0

摘要

体外受精的效率有限,部分原因是一小部分回收的卵母细胞不能受精。准确评估它们的质量可以显著提高体外受精效率,这需要更好地了解它们的成熟是如何被破坏的。在这里,我们使用一种新的实验方法定量研究了小鼠卵母细胞及其配对颗粒细胞的超排卵与衰老之间的相互作用。我们测试了超排卵破坏卵母细胞成熟的假设,揭示了在强制激素刺激下转录水平上关键的细胞间通讯通路失调。我们进一步证明颗粒细胞转录标记物可以预测相关卵母细胞的早期发育潜力。通过使用自然排卵的老年小鼠作为非刺激参考,我们发现衰老和超排卵失调了相似的基因,并相互作用。通过比较小鼠和人类颗粒细胞的转录反应,我们发现年龄相关的激素反应和细胞周期通路失调是共享的,尽管其他通路存在实质性差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Granulosa cell transcription is similarly impacted by superovulation and aging and predicts early embryonic trajectories

Granulosa cell transcription is similarly impacted by superovulation and aging and predicts early embryonic trajectories

In vitro fertilization efficiency is limited in part because a fraction of retrieved oocytes fails to fertilize. Accurately evaluating their quality could significantly improve in vitro fertilization efficiency, which would require better understanding how their maturation may be disrupted. Here, we quantitatively investigate the interplay between superovulation and aging in mouse oocytes and their paired granulosa cells using a newly adapted experimental methodology. We test the hypothesis that superovulation disrupts oocyte maturation, revealing the key intercellular communication pathways dysregulated at the transcriptional level by forced hormonal stimulation. We further demonstrate that granulosa cell transcriptional markers can prospectively predict an associated oocyte’s early developmental potential. By using naturally ovulated old mice as a non-stimulated reference, we show that aging and superovulation dysregulate similar genes and interact with each other. By comparing mice and human transcriptional responses of granulosa cells, we find that age-related dysregulation of hormonal responses and cell cycle pathways are shared, though substantial divergence exists in other pathways.

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来源期刊
Nature Communications
Nature Communications Biological Science Disciplines-
CiteScore
24.90
自引率
2.40%
发文量
6928
审稿时长
3.7 months
期刊介绍: Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
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