{"title":"Shotgun宏基因组揭示除草剂对稻田土壤抗菌素耐药性和污染物降解的影响","authors":"Laliteshwari Bhardwaj, Anand Kumar Pandey, Bhavana Pandey, Suresh Kumar Dubey","doi":"10.1007/s11270-025-07988-y","DOIUrl":null,"url":null,"abstract":"<div><p>Microbes are the most sensitive component of soil, and they are affected by any change in the soil properties caused by land use patterns and the application of pesticides. Exposure to antibiotics and pesticides results in the development of tolerant/degrading and antibiotic-resistant soil microbiomes that can flourish antimicrobial resistance (AMR) in other biota. Therefore, analyzing the emergence of AMR in soil due to anthropogenic activities is a prime concern. Due to comprehensiveness, high resolution, and versatility, a whole metagenome (shotgun) based study was conducted for the assessment of wide range of AMR and pollutant degradation pathway genes in rice field soils under the influence of land use (tillage) changes and herbicide (pendimethalin) application. In the zero tillage herbicide treatment versus zero tillage control groups, beta-lactam, vancomycin, and cationic antimicrobial peptides were the most significantly affected AMR genes, with fold change of 1.19, 1.20, and 1.31, respectively. Similarly, the most affected degradation pathways genes were of dioxin degradation, phosphonate/phosphinate metabolism, atrazine degradation, and benzoate degradation with fold change of 4.00, 2.00, 1.75, and 1.18, respectively. Overall, the study reveals an increase in AMR and pollutant degradation ability of soil microbial flora due to tillage and herbicide treatment. Pollutant degradation will aid in restoring soil health and environmental sustainability, whereas addressing AMR signifies the need for adequate monitoring and regulations to mitigate the spread of AMR. Henceforth, the outcomes would contribute in developing sustainable agricultural practices in rice fields to reduce the AMR load and enhance the efficacy of pollutant degradation in soils.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":"236 6","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Shotgun Metagenome Reveals Herbicidal Influence on Antimicrobial Resistance and Pollutant Degradation in Rice Field Soils\",\"authors\":\"Laliteshwari Bhardwaj, Anand Kumar Pandey, Bhavana Pandey, Suresh Kumar Dubey\",\"doi\":\"10.1007/s11270-025-07988-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Microbes are the most sensitive component of soil, and they are affected by any change in the soil properties caused by land use patterns and the application of pesticides. Exposure to antibiotics and pesticides results in the development of tolerant/degrading and antibiotic-resistant soil microbiomes that can flourish antimicrobial resistance (AMR) in other biota. Therefore, analyzing the emergence of AMR in soil due to anthropogenic activities is a prime concern. Due to comprehensiveness, high resolution, and versatility, a whole metagenome (shotgun) based study was conducted for the assessment of wide range of AMR and pollutant degradation pathway genes in rice field soils under the influence of land use (tillage) changes and herbicide (pendimethalin) application. In the zero tillage herbicide treatment versus zero tillage control groups, beta-lactam, vancomycin, and cationic antimicrobial peptides were the most significantly affected AMR genes, with fold change of 1.19, 1.20, and 1.31, respectively. Similarly, the most affected degradation pathways genes were of dioxin degradation, phosphonate/phosphinate metabolism, atrazine degradation, and benzoate degradation with fold change of 4.00, 2.00, 1.75, and 1.18, respectively. Overall, the study reveals an increase in AMR and pollutant degradation ability of soil microbial flora due to tillage and herbicide treatment. Pollutant degradation will aid in restoring soil health and environmental sustainability, whereas addressing AMR signifies the need for adequate monitoring and regulations to mitigate the spread of AMR. Henceforth, the outcomes would contribute in developing sustainable agricultural practices in rice fields to reduce the AMR load and enhance the efficacy of pollutant degradation in soils.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":808,\"journal\":{\"name\":\"Water, Air, & Soil Pollution\",\"volume\":\"236 6\",\"pages\":\"\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-04-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Water, Air, & Soil Pollution\",\"FirstCategoryId\":\"6\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11270-025-07988-y\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water, Air, & Soil Pollution","FirstCategoryId":"6","ListUrlMain":"https://link.springer.com/article/10.1007/s11270-025-07988-y","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Shotgun Metagenome Reveals Herbicidal Influence on Antimicrobial Resistance and Pollutant Degradation in Rice Field Soils
Microbes are the most sensitive component of soil, and they are affected by any change in the soil properties caused by land use patterns and the application of pesticides. Exposure to antibiotics and pesticides results in the development of tolerant/degrading and antibiotic-resistant soil microbiomes that can flourish antimicrobial resistance (AMR) in other biota. Therefore, analyzing the emergence of AMR in soil due to anthropogenic activities is a prime concern. Due to comprehensiveness, high resolution, and versatility, a whole metagenome (shotgun) based study was conducted for the assessment of wide range of AMR and pollutant degradation pathway genes in rice field soils under the influence of land use (tillage) changes and herbicide (pendimethalin) application. In the zero tillage herbicide treatment versus zero tillage control groups, beta-lactam, vancomycin, and cationic antimicrobial peptides were the most significantly affected AMR genes, with fold change of 1.19, 1.20, and 1.31, respectively. Similarly, the most affected degradation pathways genes were of dioxin degradation, phosphonate/phosphinate metabolism, atrazine degradation, and benzoate degradation with fold change of 4.00, 2.00, 1.75, and 1.18, respectively. Overall, the study reveals an increase in AMR and pollutant degradation ability of soil microbial flora due to tillage and herbicide treatment. Pollutant degradation will aid in restoring soil health and environmental sustainability, whereas addressing AMR signifies the need for adequate monitoring and regulations to mitigate the spread of AMR. Henceforth, the outcomes would contribute in developing sustainable agricultural practices in rice fields to reduce the AMR load and enhance the efficacy of pollutant degradation in soils.
期刊介绍：
Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments.
Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation.
Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.
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