rnai介导的伸出翼(hold -out wing, HOW)基因敲低抑制白背飞虱翅膀扩张

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY
Gui-yun Long , Zhao Wang , Dao-chao Jin , Hong Yang , Sheng-jiang Yang , Cao Zhou , Qing-hui Zeng
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引用次数: 0

摘要

白背飞虱(Sogatella furcifera)是水稻的主要迁徙害虫,对各种化学处理具有抗性。发育调控基因“伸出翼(hold -out wing, HOW)”基因可作为RNA干扰(RNAi)农药的潜在靶点。本研究通过rnai介导的基因沉默,分析了SfHOW的时空表达模式和生物学功能。SfHOW基因全长628 bp,编码196个氨基酸。SfHOW在3龄若虫中高表达,其次是5龄若虫。在不同组织中,SfHOW在表皮表达量最高,翅膀和腿部表达量较低,脂肪体和肠道表达量最低。rnai介导的SfHOW沉默导致其转录水平显著降低,导致66.8%的死亡率。此外,62.0%的羽化成虫羽翼呈折叠和卷曲状,表明SfHOW对羽化成虫羽翼的扩张起着至关重要的作用。SfHOW沉默后,S. furcifera Apterous (SfAp)的表达显著降低,提示SfHOW可能通过调节SfAp来调节翅膀扩张,从而调节Dpp基因的表达,参与翅膀扩张的调控。本研究为开发基于rnai的水稻害虫防治农药提供了新的靶点,并加深了对半翅目昆虫翅膀发育的分子认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
RNAi-mediated held-out wing (HOW) gene knockdown inhibits wing expansion of white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae)
The white-backed planthopper (Sogatella furcifera), a major migratory pest of rice, has resistance to various chemical treatments. The developmental regulation gene “held-out wing (HOW)” gene can serve as a potential target for RNA interference (RNAi) pesticides. Here, we analyzed spatiotemporal expression pattern and biological function of SfHOW by RNAi-mediated gene silencing. The SfHOW gene contains 628 bp and encodes 196 amino acids. SfHOW was highly expressed in 3rd instar nymphs, followed by 5th instar nymphs. In different tissues, SfHOW is most abundantly expressed in epidermis, with lower levels in wings and legs, and the least expression observed in fat body and gut. RNAi-mediated silencing of SfHOW resulted in a marked decrease in its transcription levels, leading to mortality of 66.8 %. Additionally, 62.0 % emerged adults exhibited folded and curled wings, indicating that SfHOW is crucial for wing expansion of S. furcifera. Following SfHOW silencing, significant reductions in the expression of S. furcifera Apterous (SfAp), suggesting that SfHOW may regulate wing expansion by modulating SfAp, so as to regulate the expression of Dpp gene to participate in the regulation of expansion. This study identified a new target for the development of RNAi-based pesticides for rice pest control and enhances molecular understanding of wing development in Hemipteran insects.
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来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
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