Gui-yun Long , Zhao Wang , Dao-chao Jin , Hong Yang , Sheng-jiang Yang , Cao Zhou , Qing-hui Zeng
{"title":"rnai介导的伸出翼(hold -out wing, HOW)基因敲低抑制白背飞虱翅膀扩张","authors":"Gui-yun Long , Zhao Wang , Dao-chao Jin , Hong Yang , Sheng-jiang Yang , Cao Zhou , Qing-hui Zeng","doi":"10.1016/j.jinsphys.2025.104810","DOIUrl":null,"url":null,"abstract":"<div><div>The white-backed planthopper (<em>Sogatella furcifera</em>), a major migratory pest of rice, has resistance to various chemical treatments. The developmental regulation gene “<em>held-out wing</em> (<em>HOW</em>)” gene can serve as a potential target for RNA interference (RNAi) pesticides. Here, we analyzed spatiotemporal expression pattern and biological function of <em>SfHOW</em> by RNAi-mediated gene silencing. The <em>SfHOW</em> gene contains 628 bp and encodes 196 amino acids. <em>SfHOW</em> was highly expressed in 3<sup>rd</sup> instar nymphs, followed by 5<sup>th</sup> instar nymphs. In different tissues, <em>SfHOW</em> is most abundantly expressed in epidermis, with lower levels in wings and legs, and the least expression observed in fat body and gut. RNAi-mediated silencing of <em>SfHOW</em> resulted in a marked decrease in its transcription levels, leading to mortality of 66.8 %. Additionally, 62.0 % emerged adults exhibited folded and curled wings, indicating that <em>SfHOW</em> is crucial for wing expansion of <em>S. furcifera</em>. Following <em>SfHOW</em> silencing, significant reductions in the expression of <em>S. furcifera Apterous</em> (<em>SfAp</em>), suggesting that <em>SfHOW</em> may regulate wing expansion by modulating <em>SfAp,</em> so as to regulate the expression of <em>Dpp</em> gene to participate in the regulation of expansion. This study identified a new target for the development of RNAi-based pesticides for rice pest control and enhances molecular understanding of wing development in Hemipteran insects.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104810"},"PeriodicalIF":2.3000,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"RNAi-mediated held-out wing (HOW) gene knockdown inhibits wing expansion of white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae)\",\"authors\":\"Gui-yun Long , Zhao Wang , Dao-chao Jin , Hong Yang , Sheng-jiang Yang , Cao Zhou , Qing-hui Zeng\",\"doi\":\"10.1016/j.jinsphys.2025.104810\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The white-backed planthopper (<em>Sogatella furcifera</em>), a major migratory pest of rice, has resistance to various chemical treatments. The developmental regulation gene “<em>held-out wing</em> (<em>HOW</em>)” gene can serve as a potential target for RNA interference (RNAi) pesticides. Here, we analyzed spatiotemporal expression pattern and biological function of <em>SfHOW</em> by RNAi-mediated gene silencing. The <em>SfHOW</em> gene contains 628 bp and encodes 196 amino acids. <em>SfHOW</em> was highly expressed in 3<sup>rd</sup> instar nymphs, followed by 5<sup>th</sup> instar nymphs. In different tissues, <em>SfHOW</em> is most abundantly expressed in epidermis, with lower levels in wings and legs, and the least expression observed in fat body and gut. RNAi-mediated silencing of <em>SfHOW</em> resulted in a marked decrease in its transcription levels, leading to mortality of 66.8 %. Additionally, 62.0 % emerged adults exhibited folded and curled wings, indicating that <em>SfHOW</em> is crucial for wing expansion of <em>S. furcifera</em>. Following <em>SfHOW</em> silencing, significant reductions in the expression of <em>S. furcifera Apterous</em> (<em>SfAp</em>), suggesting that <em>SfHOW</em> may regulate wing expansion by modulating <em>SfAp,</em> so as to regulate the expression of <em>Dpp</em> gene to participate in the regulation of expansion. This study identified a new target for the development of RNAi-based pesticides for rice pest control and enhances molecular understanding of wing development in Hemipteran insects.</div></div>\",\"PeriodicalId\":16189,\"journal\":{\"name\":\"Journal of insect physiology\",\"volume\":\"163 \",\"pages\":\"Article 104810\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-04-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of insect physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022191025000642\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of insect physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022191025000642","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
The white-backed planthopper (Sogatella furcifera), a major migratory pest of rice, has resistance to various chemical treatments. The developmental regulation gene “held-out wing (HOW)” gene can serve as a potential target for RNA interference (RNAi) pesticides. Here, we analyzed spatiotemporal expression pattern and biological function of SfHOW by RNAi-mediated gene silencing. The SfHOW gene contains 628 bp and encodes 196 amino acids. SfHOW was highly expressed in 3rd instar nymphs, followed by 5th instar nymphs. In different tissues, SfHOW is most abundantly expressed in epidermis, with lower levels in wings and legs, and the least expression observed in fat body and gut. RNAi-mediated silencing of SfHOW resulted in a marked decrease in its transcription levels, leading to mortality of 66.8 %. Additionally, 62.0 % emerged adults exhibited folded and curled wings, indicating that SfHOW is crucial for wing expansion of S. furcifera. Following SfHOW silencing, significant reductions in the expression of S. furcifera Apterous (SfAp), suggesting that SfHOW may regulate wing expansion by modulating SfAp, so as to regulate the expression of Dpp gene to participate in the regulation of expansion. This study identified a new target for the development of RNAi-based pesticides for rice pest control and enhances molecular understanding of wing development in Hemipteran insects.
期刊介绍:
All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.