{"title":"衰老可以缓解柑桔皮多糖对EGCG抑制α-淀粉酶作用的负面影响","authors":"Gang Xu , Jingyun Zhao , Kaixin Shi , Siyi Pan","doi":"10.1016/j.foodhyd.2025.111403","DOIUrl":null,"url":null,"abstract":"<div><div>Aged citrus peel (Chenpi) is traditionally consumed with tea, providing unique flavors and potential health benefits. The structural changes of Chenpi polysaccharides (CP) during aging may significantly influence their interactions with tea polyphenols, particularly epigallocatechin gallate (EGCG), thereby contributing to the regulation of postprandial blood glucose levels. This study systematically investigated the regulatory effects of CP at different aging stages on the binding behavior of EGCG with α-amylase (PPA) and its <em>in vitro</em> hypoglycemic activity. The results of the study demonstrated that CP significantly interfered with the inhibitory effect of EGCG on PPA in a time-dependent manner. CP5 (aged 5 years) exhibited the weakest interference, alleviating over 40 % of the inhibition loss observed in early aging. However, prolonged aging beyond 10 years gradually reintroduced the interference effect. CP did not alter EGCG's competitive inhibition mechanism but reduced its binding efficiency with PPA, likely by competing for hydrogen bond interactions. Moreover, CP significantly mitigated EGCG-induced enzyme fluorescence quenching, conformational unfolding, and thermal stability loss. The interaction strength between CP and EGCG followed the order of CP1 > CP15 > CP10 > CP5, consistent with enzyme activity results. This study revealed that CP regulated postprandial glucose control by dynamically modulating the interaction between EGCG and PPA, providing valuable insights for optimizing the health benefits of Chenpi-tea systems.</div></div>","PeriodicalId":320,"journal":{"name":"Food Hydrocolloids","volume":"167 ","pages":"Article 111403"},"PeriodicalIF":11.0000,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Aging alleviates the negative impact of citrus peel polysaccharides on the inhibitory effect of EGCG against α-Amylase\",\"authors\":\"Gang Xu , Jingyun Zhao , Kaixin Shi , Siyi Pan\",\"doi\":\"10.1016/j.foodhyd.2025.111403\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Aged citrus peel (Chenpi) is traditionally consumed with tea, providing unique flavors and potential health benefits. The structural changes of Chenpi polysaccharides (CP) during aging may significantly influence their interactions with tea polyphenols, particularly epigallocatechin gallate (EGCG), thereby contributing to the regulation of postprandial blood glucose levels. This study systematically investigated the regulatory effects of CP at different aging stages on the binding behavior of EGCG with α-amylase (PPA) and its <em>in vitro</em> hypoglycemic activity. The results of the study demonstrated that CP significantly interfered with the inhibitory effect of EGCG on PPA in a time-dependent manner. CP5 (aged 5 years) exhibited the weakest interference, alleviating over 40 % of the inhibition loss observed in early aging. However, prolonged aging beyond 10 years gradually reintroduced the interference effect. CP did not alter EGCG's competitive inhibition mechanism but reduced its binding efficiency with PPA, likely by competing for hydrogen bond interactions. Moreover, CP significantly mitigated EGCG-induced enzyme fluorescence quenching, conformational unfolding, and thermal stability loss. The interaction strength between CP and EGCG followed the order of CP1 > CP15 > CP10 > CP5, consistent with enzyme activity results. This study revealed that CP regulated postprandial glucose control by dynamically modulating the interaction between EGCG and PPA, providing valuable insights for optimizing the health benefits of Chenpi-tea systems.</div></div>\",\"PeriodicalId\":320,\"journal\":{\"name\":\"Food Hydrocolloids\",\"volume\":\"167 \",\"pages\":\"Article 111403\"},\"PeriodicalIF\":11.0000,\"publicationDate\":\"2025-04-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Hydrocolloids\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0268005X25003637\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Hydrocolloids","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0268005X25003637","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Aging alleviates the negative impact of citrus peel polysaccharides on the inhibitory effect of EGCG against α-Amylase
Aged citrus peel (Chenpi) is traditionally consumed with tea, providing unique flavors and potential health benefits. The structural changes of Chenpi polysaccharides (CP) during aging may significantly influence their interactions with tea polyphenols, particularly epigallocatechin gallate (EGCG), thereby contributing to the regulation of postprandial blood glucose levels. This study systematically investigated the regulatory effects of CP at different aging stages on the binding behavior of EGCG with α-amylase (PPA) and its in vitro hypoglycemic activity. The results of the study demonstrated that CP significantly interfered with the inhibitory effect of EGCG on PPA in a time-dependent manner. CP5 (aged 5 years) exhibited the weakest interference, alleviating over 40 % of the inhibition loss observed in early aging. However, prolonged aging beyond 10 years gradually reintroduced the interference effect. CP did not alter EGCG's competitive inhibition mechanism but reduced its binding efficiency with PPA, likely by competing for hydrogen bond interactions. Moreover, CP significantly mitigated EGCG-induced enzyme fluorescence quenching, conformational unfolding, and thermal stability loss. The interaction strength between CP and EGCG followed the order of CP1 > CP15 > CP10 > CP5, consistent with enzyme activity results. This study revealed that CP regulated postprandial glucose control by dynamically modulating the interaction between EGCG and PPA, providing valuable insights for optimizing the health benefits of Chenpi-tea systems.
期刊介绍:
Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication.
The main areas of interest are:
-Chemical and physicochemical characterisation
Thermal properties including glass transitions and conformational changes-
Rheological properties including viscosity, viscoelastic properties and gelation behaviour-
The influence on organoleptic properties-
Interfacial properties including stabilisation of dispersions, emulsions and foams-
Film forming properties with application to edible films and active packaging-
Encapsulation and controlled release of active compounds-
The influence on health including their role as dietary fibre-
Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes-
New hydrocolloids and hydrocolloid sources of commercial potential.
The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.