辣椒（Capsicum annuum L.） PLATZ基因家族的分子谱分析及capplatz6的过表达增强了辣椒对炭疽病菌的抗性。

IF 3.3 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-04-15 DOI:10.1016/j.pmpp.2025.102700

Swarna Manjari Mishra , Sravya Sruti Tamarapalli , Rukmini Mishra , Raj Kumar Joshi

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引用次数: 0

摘要

植物富含at序列和锌结合（PLATZ）转录因子广泛参与植物的生长发育和逆境响应。在本研究中，我们对辣椒（Capsicum annuum）的PLATZ基因家族进行了全基因组分析，并研究了其对防御激素和病原体感染的响应功能。辣椒基因组包含12个PLATZ基因，可分为4组，每组具有相似的内含子-外显子结构和基序组成。对PLATZ基因启动子区域的顺式元件分析表明，存在生长发育、光信号、植物激素和胁迫响应蛋白的结合位点。capplatz1、2、3、6、8和9对防御激素水杨酸（SA）的差异表达；SA和JA对茉莉酸（JA）和乙烯（ET）的诱导作用显著，ET对其抑制作用显著。对抗性辣椒品种Punjab Lal （PL）和敏感品系Arka Lohit （AL）经炭黑病菌（cot）处理后的PLATZ基因进行qRT-PCR分析表明，在不亲和的互作条件下，capplatz3和CaPLATZ6被显著诱导。此外，来自过表达capplatz6的敏感系AL的转基因辣椒植株对炭疽病的抗性增强，表现为真菌生长减少，防御反应基因表达上调。这些结果为PLATZ基因的表达和可能的作用及其在工程抗逆性作物中的潜在应用提供了新的见解。

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Molecular profiling of PLATZ gene family in pepper (Capsicum annuum L.) and overexpression of CaPLATZ6 confers enhanced resistance to anthracnose pathogen, Colletotrichum truncatum L.

The plant AT-rich sequence and zinc-binding (PLATZ) transcription factors are widely involved in growth, development and stress response. In the present study, we have conducted a genome-wide profiling of PLATZ gene family in pepper (Capsicum annuum) and examined their functions in response to defense hormones and pathogen infection. The pepper genome contains 12 PLATZ genes that could be classified into four groups with each group possessing similar intron-exon structures and motif composition. Cis-element analysis of the promoter regions of the PLATZ genes showed the presence of binding site for proteins responsive to growth and development, light signaling, phytohormones and stress response. CaPLATZ1, 2, 3, 6, 8 and 9 were differentially expressed in response to defense hormones salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) with CaPLATZ6 significantly induced with SA and JA and repressed with ET. qRT-PCR analysis of the PLATZ genes in the resistant pepper cultivar Punjab Lal (PL) and sensitive line Arka Lohit (AL) post treatment with anthracnose pathogen Colletotrichum truncatum (cot) showed that CaPLATZ3 and CaPLATZ6 were significantly induced under incompatible resistant interaction. Furthermore, transgenic pepper plants from the sensitive line AL overexpressing CaPLATZ6 showed enhanced resistance to anthracnose, as revealed by decreased fungal growth and up-regulated expression of defense-responsive genes. These results indicate fresh insights about the expression and likely role of PLATZ genes and their potential application in engineering stress tolerant crops.

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.