Minghui Xie , Yongzhi Zhong , Lulu Lin , Guangling Zhang , Ning Wei , Feng Zhang , Haoliang Chen
{"title":"狐尾夜蛾幼虫对烟草的适应性和解毒能力的综合转录组和代谢组分析","authors":"Minghui Xie , Yongzhi Zhong , Lulu Lin , Guangling Zhang , Ning Wei , Feng Zhang , Haoliang Chen","doi":"10.1016/j.jinsphys.2025.104800","DOIUrl":null,"url":null,"abstract":"<div><div><em>Spodoptera frugiperda</em> is among the most significant pests affecting food crops globally. Our findings indicate that the mortality rates of 1st and 2nd instar larvae are significantly higher than those of 3rd instar and older larvae following tobacco consumption. In this study, we employed integrated transcriptomic and metabolomic analyses to investigate the changes in gene expression and metabolic processes in 2nd and 3rd instar larvae after consuming tobacco and maize. Transcriptome analysis revealed that the majority of differentially expressed genes (DEGs) involved in xenobiotic biodegradation and metabolism were upregulated, particularly cytochrome P450s. Metabolomic analysis identified alkaloid metabolites in the bodies of larvae that had fed on tobacco. The 3rd instar larvae that consumed tobacco exhibited increased production of metabolites via cytochrome P450. Correlation analysis of the transcriptome and metabolome demonstrated that, when comparing the 3rd instar larvae fed on tobacco to those fed on maize, both DEGs and differentially accumulated metabolites (DAMs) shared pathways related to cytochrome P450, fatty acid metabolism, and glutathione metabolism. Transcriptome and metabolome analysis shows cytochrome P450 play an important role in the detoxification and adaptability of <em>S. frugiperda</em> larvae to tobacco. This study provides a preliminary explanation of the detoxification metabolism and adaptive mechanisms of <em>S. frugiperda</em> larvae in response to tobacco.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104800"},"PeriodicalIF":2.3000,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comprehensive transcriptome and metabolome analysis of the adaptability and detoxification ability of Spodoptera frugiperda larvae to tobacco\",\"authors\":\"Minghui Xie , Yongzhi Zhong , Lulu Lin , Guangling Zhang , Ning Wei , Feng Zhang , Haoliang Chen\",\"doi\":\"10.1016/j.jinsphys.2025.104800\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Spodoptera frugiperda</em> is among the most significant pests affecting food crops globally. Our findings indicate that the mortality rates of 1st and 2nd instar larvae are significantly higher than those of 3rd instar and older larvae following tobacco consumption. In this study, we employed integrated transcriptomic and metabolomic analyses to investigate the changes in gene expression and metabolic processes in 2nd and 3rd instar larvae after consuming tobacco and maize. Transcriptome analysis revealed that the majority of differentially expressed genes (DEGs) involved in xenobiotic biodegradation and metabolism were upregulated, particularly cytochrome P450s. Metabolomic analysis identified alkaloid metabolites in the bodies of larvae that had fed on tobacco. The 3rd instar larvae that consumed tobacco exhibited increased production of metabolites via cytochrome P450. Correlation analysis of the transcriptome and metabolome demonstrated that, when comparing the 3rd instar larvae fed on tobacco to those fed on maize, both DEGs and differentially accumulated metabolites (DAMs) shared pathways related to cytochrome P450, fatty acid metabolism, and glutathione metabolism. Transcriptome and metabolome analysis shows cytochrome P450 play an important role in the detoxification and adaptability of <em>S. frugiperda</em> larvae to tobacco. This study provides a preliminary explanation of the detoxification metabolism and adaptive mechanisms of <em>S. frugiperda</em> larvae in response to tobacco.</div></div>\",\"PeriodicalId\":16189,\"journal\":{\"name\":\"Journal of insect physiology\",\"volume\":\"163 \",\"pages\":\"Article 104800\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-04-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of insect physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S002219102500054X\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of insect physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S002219102500054X","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Comprehensive transcriptome and metabolome analysis of the adaptability and detoxification ability of Spodoptera frugiperda larvae to tobacco
Spodoptera frugiperda is among the most significant pests affecting food crops globally. Our findings indicate that the mortality rates of 1st and 2nd instar larvae are significantly higher than those of 3rd instar and older larvae following tobacco consumption. In this study, we employed integrated transcriptomic and metabolomic analyses to investigate the changes in gene expression and metabolic processes in 2nd and 3rd instar larvae after consuming tobacco and maize. Transcriptome analysis revealed that the majority of differentially expressed genes (DEGs) involved in xenobiotic biodegradation and metabolism were upregulated, particularly cytochrome P450s. Metabolomic analysis identified alkaloid metabolites in the bodies of larvae that had fed on tobacco. The 3rd instar larvae that consumed tobacco exhibited increased production of metabolites via cytochrome P450. Correlation analysis of the transcriptome and metabolome demonstrated that, when comparing the 3rd instar larvae fed on tobacco to those fed on maize, both DEGs and differentially accumulated metabolites (DAMs) shared pathways related to cytochrome P450, fatty acid metabolism, and glutathione metabolism. Transcriptome and metabolome analysis shows cytochrome P450 play an important role in the detoxification and adaptability of S. frugiperda larvae to tobacco. This study provides a preliminary explanation of the detoxification metabolism and adaptive mechanisms of S. frugiperda larvae in response to tobacco.
期刊介绍:
All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.