基于萘酰亚胺和菲罗啉的新型大斯托克斯位移荧光探针对银离子的特异性检测及其在活细胞成像中的应用

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-11 DOI:10.1016/j.saa.2025.126222

Chenglu Zhang, Jinghao Guo, Yang Zhang, Cui Liu

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引用次数: 0

摘要

银离子（Ag+）被广泛应用于工业和日常生活中。为了实现对 Ag+ 的特异性和灵敏度检测，本研究开发了一种基于萘二甲酰亚胺-菲罗啉的新型荧光探针 NP。NP 的发射波长为 580 nm，并表现出较大的斯托克斯位移（190 nm），对 Ag+ 具有淬灭荧光响应。NP 可灵敏地检测 Ag+，检测限低至 24.6 nM。特别是，NP 能有效检测真实水样中的 Ag+。此外，NP 的毒性很低，已成功应用于 Hela 细胞中的 Ag+ 成像。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A novel fluorescent probe with large stokes shift based on naphthalimide and phenanthroline for specific detection of Ag+ and its application in living cells imaging

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A novel fluorescent probe with large stokes shift based on naphthalimide and phenanthroline for specific detection of Ag+ and its application in living cells imaging

Silver ions (Ag⁺) are widely used in industry and daily life. To achieve the specific and sensitive detection of Ag⁺, a novel fluorescent probe NP based on naphthalimide-phenanthroline was developed in this study. NP has an emission wavelength of 580 nm and exhibits a large Stokes shift (190 nm), showing a quenching fluorescence response to Ag⁺. NP can sensitively detect Ag⁺ with a detection limit as low as 24.6 nM. The response time is only 110 s. In particular, NP demonstrates effective detection of Ag⁺ in real water samples. In addition, NP exhibits low toxicity and has been successfully applied for Ag⁺ imaging in Hela cells.

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.